重组
RabMAb

Anti-DP1抗体[EPR4959(2)] (ab124678)

概述

  • 产品名称
    Anti-DP1抗体[EPR4959(2)]
    参阅全部 DP1 一抗
  • 描述
    兔单克隆抗体[EPR4959(2)] to DP1
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WB, Flow Cytmore details
    不适用于: ICC,IHC-P or IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide within Human DP1 aa 350-450. The exact sequence is proprietary.

  • 阳性对照
    • Raji cell lysate; HeLa cells.
  • 常规说明

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

性能

应用

Our Abpromise guarantee covers the use of ab124678 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/10000 - 1/50000. Detects a band of approximately 49 kDa (predicted molecular weight: 45 kDa).
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • 应用说明
    Is unsuitable for ICC,IHC-P or IP.
  • 靶标

    • 功能
      Can stimulate E2F-dependent transcription. Binds DNA cooperatively with E2F family members through the E2 recognition site, 5'-TTTC[CG]CGC-3', found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DP2/E2F complex functions in the control of cell-cycle progression from G1 to S phase. The E2F-1/DP complex appears to mediate both cell proliferation and apoptosis.
    • 组织特异性
      Highest levels in muscle. Also expressed in brain, placenta, liver and kidney. Lower levels in lung and pancreas. Not detected in heart.
    • 序列相似性
      Belongs to the E2F/DP family.
    • 翻译后修饰
      Phosphorylation by E2F-1-bound cyclin A-CDK2, in the S phase, inhibits E2F-mediated DNA binding and transactivation.
    • 细胞定位
      Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • DP 1 antibody
      • DP1 antibody
      • DRTF1 antibody
      • DRTF1-polypeptide 1 antibody
      • E2F dimerization partner 1 antibody
      • E2F related transcription factor antibody
      • TFDP1 antibody
      • TFDP1_HUMAN antibody
      • Transcription factor Dp-1 antibody
      • Transcription factor sequence-specific, DRTF1 antibody
      see all

    图片

    • ab124678 at 1/10 dilution staining DP1 in permeabilized HeLa cells by Flow cytometry (red). Rabbit IgG negative control (green).
    • Anti-DP1 antibody [EPR4959(2)] (ab124678) at 1/10000 dilution + Raji cell lysate at 10 µg

      Secondary
      Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size: 45 kDa
      Observed band size: 49 kDa (why is the actual band size different from the predicted?)

    文献

    ab124678 has not yet been referenced specifically in any publications.

    客户评价及客户问答

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HAP1 cells)
    Gel Running Conditions
    Reduced Denaturing (7%)
    Loading amount
    20 µg
    Specification
    HAP1 cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    提交于 Jun 21 2018

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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