Anti-Doublecortin抗体- Neuronal Marker (ab77450)

概述

  • 产品名称Anti-Doublecortin抗体- Neuronal Marker
    参阅全部 Doublecortin 一抗
  • 描述
    兔多克隆抗体to Doublecortin - Neuronal Marker
  • 经测试应用适用于: WB, ICC/IF, IHC-FoFrmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Chicken
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 300 to the C-terminus of Human Doublecortin.

    (Peptide available as ab19804.)

  • 阳性对照
    • This antibody gave a positive signal in Mouse 0 Day Brain Tissue lysate
  • 常规说明ab77450 is raised using the same immunogen as ab18723. Batches sold under ab77450 have passed QC testing in Western Blot and ICC/IF. In our hands, this product did not work in IHC-P. For further information please contact our Customer Services team.

性能

应用

Our Abpromise guarantee covers the use of ab77450 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 40-45 kDa).Can be blocked with Human Doublecortin peptide (ab19804).
ICC/IF Use a concentration of 1 - 5 µg/ml.
IHC-FoFr 1/1000.

靶标

  • 功能Seems to be required for initial steps of neuronal dispersion and cortex lamination during cerebral cortex development. May act by competing with the putative neuronal protein kinase DCAMKL1 in binding to a target protein. May in that way participate in a signaling pathway that is crucial for neuronal interaction before and during migration, possibly as part of a calcium ion-dependent signal transduction pathway. May be part with LIS-1 of an overlapping, but distinct, signaling pathways that promote neuronal migration.
  • 组织特异性Highly expressed in neuronal cells of fetal brain (in the majority of cells of the cortical plate, intermediate zone and ventricular zone), but not expressed in other fetal tissues. In the adult, highly expressed in the brain frontal lobe, but very low expression in other regions of brain, and not detected in heart, placenta, lung, liver, skeletal muscles, kidney and pancreas.
  • 疾病相关Defects in DCX are the cause of lissencephaly X-linked type 1 (LISX1) [MIM:300067]; also called X-LIS or LIS. LISX1 is a classic lissencephaly characterized by mental retardation and seizures that are more severe in male patients. Affected boys show an abnormally thick cortex with absent or severely reduced gyri. Clinical manifestations include feeding problems, abnormal muscular tone, seizures and severe to profound psychomotor retardation. Female patients display a less severe phenotype referred to as 'doublecortex'.
    Defects in DCX are the cause of subcortical band heterotopia X-linked (SBHX) [MIM:300067]; also known as double cortex or subcortical laminar heterotopia (SCLH). SBHX is a mild brain malformation of the lissencephaly spectrum. It is characterized by bilateral and symmetric plates or bands of gray matter found in the central white matter between the cortex and cerebral ventricles, cerebral convolutions usually appearing normal.
    Note=A chromosomal aberration involving DCX is found in lissencephaly. Translocation t(X;2)(q22.3;p25.1).
  • 序列相似性Contains 2 doublecortin domains.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DBCN antibody
    • Dbct antibody
    • DC antibody
    • DCX antibody
    • DCX_HUMAN antibody
    • Doublecortex antibody
    • Doublin antibody
    • FLJ51296 antibody
    • Lis X antibody
    • Lis-X antibody
    • Lissencephalin X antibody
    • Lissencephalin-X antibody
    • Lissencephaly X linked antibody
    • Lissencephaly X linked doublecortin antibody
    • LISX antibody
    • Neuronal migration protein doublecortin antibody
    • OTTHUMP00000023859 antibody
    • OTTHUMP00000023860 antibody
    • OTTHUMP00000216315 antibody
    • OTTHUMP00000216316 antibody
    • SCLH antibody
    • XLIS antibody
    see all

Anti-Doublecortin antibody - Neuronal Marker 图像

  • ICC/IF image of ab77450 stained SH-SY5Y cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab77450, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue).
  • Immunohistochemistical detection of Doublecortin using ab77450 on PFA perfusion-fixed free floating 30um rat brain sections. Primary Antibody diluted 1/1000 and incubated for 18 hours @ 20°C in PBS + 0.3 % Triton X100. Secondary Antibody: Goat anti-rabbit Alexa Fluor® 488 (1/1000). Excellent staining was observed in rat brain dentate gyrus neurons with ab77450.

    See Abreview

  • Anti-Doublecortin antibody - Neuronal Marker (ab77450) at 1 µg/ml + Mouse brain tissue lysate - total protein (0 days) (ab7188) at 10 µg

    Secondary
    Rabbit IgG secondary antibody (ab28446) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 40-45 kDa


    Exposure time : 1 minute

  • Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 40-45 kDa


    Exposure time : 10 seconds

Anti-Doublecortin antibody - Neuronal Marker (ab77450)参考文献

This product has been referenced in:
  • Shi W  et al. Transplantation of RADA16-BDNF peptide scaffold with human umbilical cord mesenchymal stem cells forced with CXCR4 and activated astrocytes for repair of traumatic brain injury. Acta Biomater 45:247-261 (2016). IF . Read more (PubMed: 27592818) »
  • Kremer T  et al. Analysis of adult neurogenesis: evidence for a prominent "non-neurogenic" DCX-protein pool in rodent brain. PLoS One 8:e59269 (2013). Read more (PubMed: 23690918) »

See all 5 Publications for this product

Product Wall

This is a complaint from earlier this year. I had been awaiting some further information, however I have not received it. I am so sorry that I have not followed up with you and the customer meanwhile on this problem. Do you have any updates? How has th...

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ab77450 is raised using the same immunogen as ab18723 and therefore the products are essentially identical. The only difference between these products is that batches sold under ab77450 have passed QC testing in Western Blot and ICC/IF however, in our ...

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Thank you for kindly confirming these details. I am sorry these vials of antibody have not worked for this customer.

As requested, we are pleased to arrange a free of charge replacement. I can confirm that 1 vial each of ab6789, ab72439 and ...

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Thank you for your message and for providing this further information.

I am sorry to hear the suggestions made have not improved the results on this occasion.

I appreciate the time the customer has spent on these experiments, and ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Dorsal Root Ganglia (DRG) neurons)
Specification Dorsal Root Ganglia (DRG) neurons
Fixative Formaldehyde
Permeabilization Yes - tween 20
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 21°C
Username

Abcam user community

Verified customer

提交于 Nov 05 2012

Thank you for your message and I appreciate the cooperation from the customer. It is regrettable that three antibodies have not worked for them.

I look forward to hearing from you with the results of the suggestions made. I hope these will h...

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Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

The details you have kindly provided will enable us to investigate this...

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Thank you for contacting Abcam.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement.

To check the status of the ord...

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Thank you very much for your feedback concerning these two antibodies. I would like to assure you that we are not misrepresenting information. Both antibodies are polyclonal and produced using the same immunogen and were originally cons...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Rat Tissue sections (Brain sections)
Specification Brain sections
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization No
Username

Dr. Sophie Pezet

Verified customer

提交于 May 10 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"