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Our Abpromise guarantee covers the use of ab77450 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 40-45 kDa).Can be blocked with Human Doublecortin peptide (ab19804).|
|ICC/IF||Use a concentration of 1 - 5 µg/ml.|
Immunohistochemistical detection of Doublecortin using ab77450 on PFA perfusion-fixed free floating 30um rat brain sections. Primary Antibody diluted 1/1000 and incubated for 18 hours @ 20°C in PBS + 0.3 % Triton X100. Secondary Antibody: Goat anti-rabbit Alexa Fluor® 488 (1/1000). Excellent staining was observed in rat brain dentate gyrus neurons with ab77450.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"