Anti-Doublecortin抗体- Neuronal Marker (ab28941)

概述

  • 产品名称Anti-Doublecortin抗体- Neuronal Marker
    参阅全部 Doublecortin 一抗
  • 描述
    兔多克隆抗体to Doublecortin - Neuronal Marker
  • 特异性ab28941 detects mouse doublecortin protein at ~43kDa on mouse brain lysate as well ~70 kDa and 75 kDa bands which we believe to be doublecortin in complex with one of a number of associating proteins such as p35, p25 or cdk5. It is possible that reducing the lysate samples further will disassociate these larger ~70 kDa bands into their components. Both the 43 and 70kDa bands are completely blocked following preincubation with the doublecortin immunising peptide (ab19803), however the third ~75kDa band is only slightly blocked by peptide preincubation. WB in human brain lysate demonstrates the presence of a doublecortin specific 53 kDa band as well as a larger ~70kDa band (data not shown).
  • 经测试应用适用于: WB, ICC/IFmore details
    不适用于: IHC-FoFr
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Chicken, Chimpanzee
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Rat Doublecortin.

    (Peptide available as ab19803.)

  • 阳性对照
    • Mouse brain lysate

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab28941 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 53, 70 kDa (predicted molecular weight: 43-53 kDa).
ICC/IF Use a concentration of 1 - 5 µg/ml.
  • 应用说明Is unsuitable for IHC-FoFr.
  • 靶标

    • 功能Seems to be required for initial steps of neuronal dispersion and cortex lamination during cerebral cortex development. May act by competing with the putative neuronal protein kinase DCAMKL1 in binding to a target protein. May in that way participate in a signaling pathway that is crucial for neuronal interaction before and during migration, possibly as part of a calcium ion-dependent signal transduction pathway. May be part with LIS-1 of an overlapping, but distinct, signaling pathways that promote neuronal migration.
    • 组织特异性Highly expressed in neuronal cells of fetal brain (in the majority of cells of the cortical plate, intermediate zone and ventricular zone), but not expressed in other fetal tissues. In the adult, highly expressed in the brain frontal lobe, but very low expression in other regions of brain, and not detected in heart, placenta, lung, liver, skeletal muscles, kidney and pancreas.
    • 疾病相关Defects in DCX are the cause of lissencephaly X-linked type 1 (LISX1) [MIM:300067]; also called X-LIS or LIS. LISX1 is a classic lissencephaly characterized by mental retardation and seizures that are more severe in male patients. Affected boys show an abnormally thick cortex with absent or severely reduced gyri. Clinical manifestations include feeding problems, abnormal muscular tone, seizures and severe to profound psychomotor retardation. Female patients display a less severe phenotype referred to as 'doublecortex'.
      Defects in DCX are the cause of subcortical band heterotopia X-linked (SBHX) [MIM:300067]; also known as double cortex or subcortical laminar heterotopia (SCLH). SBHX is a mild brain malformation of the lissencephaly spectrum. It is characterized by bilateral and symmetric plates or bands of gray matter found in the central white matter between the cortex and cerebral ventricles, cerebral convolutions usually appearing normal.
      Note=A chromosomal aberration involving DCX is found in lissencephaly. Translocation t(X;2)(q22.3;p25.1).
    • 序列相似性Contains 2 doublecortin domains.
    • 细胞定位Cytoplasm.
    • Information by UniProt
    • 数据库链接
    • 别名
      • DBCN antibody
      • Dbct antibody
      • DC antibody
      • DCX antibody
      • DCX_HUMAN antibody
      • Doublecortex antibody
      • Doublin antibody
      • FLJ51296 antibody
      • Lis X antibody
      • Lis-X antibody
      • Lissencephalin X antibody
      • Lissencephalin-X antibody
      • Lissencephaly X linked antibody
      • Lissencephaly X linked doublecortin antibody
      • LISX antibody
      • Neuronal migration protein doublecortin antibody
      • OTTHUMP00000023859 antibody
      • OTTHUMP00000023860 antibody
      • OTTHUMP00000216315 antibody
      • OTTHUMP00000216316 antibody
      • SCLH antibody
      • XLIS antibody
      see all

    Anti-Doublecortin antibody - Neuronal Marker 图像

    • ICC/IF image of ab28941 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28941, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue).
    • Doublecortin antibody - Neuronal Marker (ab28941; 5ug/ml) cytosolic and axonal staining in dorsal root ganglion explants, dissected from 16 day-old rat embryos and cultured for 6 hours or 4 days in vitro with Neurobasal Medium containing 10% fetal calf serum and B27 supplement. Immunocytochemistry: All steps were performed in PBS. Cells or explants were fixed in 4% PFA for 15min, permeabilised with 0.1% TX100 for 10min and blocked with 5% BSA, 0.1% TX100 for 45min. ab28941 was incubated at 12h in 5% BSA, 0.1% TX100 at 4°C. Preincubation of ab28941 with immunising peptide ab19803 blocked immunostaining. To-pro-3 was used as a nuclear counterstain. Treated cultures were mounted on glass coverslips with Mowiol.

    Anti-Doublecortin antibody - Neuronal Marker (ab28941)参考文献

    This product has been referenced in:
    • Head BP  et al. Inhibition of p75 neurotrophin receptor attenuates isoflurane-mediated neuronal apoptosis in the neonatal central nervous system. Anesthesiology 110:813-25 (2009). ICC/IF ; Mouse . Read more (PubMed: 19293698) »

    See 1 Publication for this product

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample Rat Tissue sections (Brain sections)
    Specification Brain sections
    Fixative Paraformaldehyde
    Antigen retrieval step None
    Permeabilization No
    Username

    Dr. Sophie Pezet

    Verified customer

    提交于 Jun 01 2010

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Rat Cell (Cultured DRG explants)
    Specification Cultured DRG explants
    Fixative Paraformaldehyde
    Blocking step BSA as blocking agent for 40 minute(s) · Concentration: 5%
    Username

    Dr. Randal Moldrich

    Verified customer

    提交于 Mar 07 2006

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"