• 产品名称Anti-Doublecortin抗体
    参阅全部 Doublecortin 一抗
  • 描述
    兔多克隆抗体to Doublecortin
  • 特异性ab61121 antibody detects endogenous levels of total Doublecortin protein.
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat
  • 免疫原

    Synthetic non-phosphopeptide derived from Human Doublecortin (isoforms 2-5 inclusive, Swiss-Prot O43602) from around the phosphorylation site of serine 297 (A-K-SP-P-G).

  • 阳性对照
    • Extracts from RAW264.7 cells.



Our Abpromise guarantee covers the use of ab61121 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 43-45 kDa).
ELISA 1/10000.


  • 功能Seems to be required for initial steps of neuronal dispersion and cortex lamination during cerebral cortex development. May act by competing with the putative neuronal protein kinase DCAMKL1 in binding to a target protein. May in that way participate in a signaling pathway that is crucial for neuronal interaction before and during migration, possibly as part of a calcium ion-dependent signal transduction pathway. May be part with LIS-1 of an overlapping, but distinct, signaling pathways that promote neuronal migration.
  • 组织特异性Highly expressed in neuronal cells of fetal brain (in the majority of cells of the cortical plate, intermediate zone and ventricular zone), but not expressed in other fetal tissues. In the adult, highly expressed in the brain frontal lobe, but very low expression in other regions of brain, and not detected in heart, placenta, lung, liver, skeletal muscles, kidney and pancreas.
  • 疾病相关Defects in DCX are the cause of lissencephaly X-linked type 1 (LISX1) [MIM:300067]; also called X-LIS or LIS. LISX1 is a classic lissencephaly characterized by mental retardation and seizures that are more severe in male patients. Affected boys show an abnormally thick cortex with absent or severely reduced gyri. Clinical manifestations include feeding problems, abnormal muscular tone, seizures and severe to profound psychomotor retardation. Female patients display a less severe phenotype referred to as 'doublecortex'.
    Defects in DCX are the cause of subcortical band heterotopia X-linked (SBHX) [MIM:300067]; also known as double cortex or subcortical laminar heterotopia (SCLH). SBHX is a mild brain malformation of the lissencephaly spectrum. It is characterized by bilateral and symmetric plates or bands of gray matter found in the central white matter between the cortex and cerebral ventricles, cerebral convolutions usually appearing normal.
    Note=A chromosomal aberration involving DCX is found in lissencephaly. Translocation t(X;2)(q22.3;p25.1).
  • 序列相似性Contains 2 doublecortin domains.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DBCN antibody
    • Dbct antibody
    • DC antibody
    • DCX antibody
    • DCX_HUMAN antibody
    • Doublecortex antibody
    • Doublin antibody
    • FLJ51296 antibody
    • Lis X antibody
    • Lis-X antibody
    • Lissencephalin X antibody
    • Lissencephalin-X antibody
    • Lissencephaly X linked antibody
    • Lissencephaly X linked doublecortin antibody
    • LISX antibody
    • Neuronal migration protein doublecortin antibody
    • OTTHUMP00000023859 antibody
    • OTTHUMP00000023860 antibody
    • OTTHUMP00000216315 antibody
    • OTTHUMP00000216316 antibody
    • SCLH antibody
    • XLIS antibody
    see all

Anti-Doublecortin antibody 图像

  • All lanes : Anti-Doublecortin antibody (ab61121) at 1/500 dilution

    Lane 1 : Extracts from RAW264.7cells
    Lane 2 : Extracts from RAW264.7cells with immunising Doublecortin peptide at 5 µg

    Lysates/proteins at 5 µg per lane.

    Predicted band size : 43-45 kDa
    Observed band size : 45 kDa (why is the actual band size different from the predicted?)

Anti-Doublecortin antibody (ab61121)参考文献

ab61121 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Mouse Tissue sections (C57BL/6 (8w), Brain, perfusion fixed 4% FA, 10 um)
Specification C57BL/6 (8w), Brain, perfusion fixed 4% FA, 10 um
Fixative Acetone
Permeabilization Yes - 0.01% Tween 20
Blocking step PBS +1% BSA +1% ovalbumine +1% goat serum as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: 22°C

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提交于 Aug 13 2009