ab121378 shows specific reactivity against target antigen.
antigen sequence, corresponding to internal sequence amino acids 70-179 of Human DOS, isoform 2.
- Human kidney tissue. U-251 MG cell and Human Liver lysate.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Information by UniProt
- Chromosome 19 open reading frame 26 antibody
- dos antibody
- DOS_HUMAN antibody
Immunocytochemistry/ Immunofluorescence - Anti-DOS antibody (ab121378)
Immunofluorescent staining of Human cell line U-2 OS shows positivity in nuclei but not nucleoli. Recommended concentration of ab121378 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DOS antibody (ab121378)
ab121378 at 1/100 dilution staining DOS in Human kidey tissue by Immunohistochemistry. This image shows cytoplasmic positivity in cells in tubules.
has not yet been referenced specifically in any publications.
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