Anti-Doppel抗体(ab23701)

概述

  • 产品名称Anti-Doppel抗体
  • 描述
    兔多克隆抗体to Doppel
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide:

    TQAANQGEF

    , corresponding to amino acids 112-120 of Human Doppel.

  • 阳性对照
    • Mouse spleen 100k x g supernatant for Western blot.

性能

应用

Our Abpromise guarantee covers the use of ab23701 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 2 µg/ml. Predicted molecular weight: 20 kDa.
IHC-P Use a concentration of 1 µg/ml.

靶标

Anti-Doppel antibody 图像

  • All lanes : Anti-Doppel antibody (ab23701) at 2 µg/ml

    Lane 1 : 27ug mouse spleen 100k x g supernatant.
    Lane 2 : 54ug mouse spleen 100k x g supernatant.


    Performed under reducing conditions.

    Predicted band size : 20 kDa
    Observed band size : 19 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 25 kDa (possible glycosylated form).

Anti-Doppel antibody (ab23701)参考文献

ab23701 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - whole (CNS)
Gel Running Conditions Reduced Denaturing (12)
Loading amount 50 µg
Specification CNS
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Nov 18 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"