Anti-Dopamine Transporter抗体[hDAT-NT] (ab5990)

概述

  • 产品名称
    Anti-Dopamine Transporter抗体[hDAT-NT]
    参阅全部 Dopamine Transporter 一抗
  • 描述
    大鼠单克隆抗体[hDAT-NT] to Dopamine Transporter
  • 特异性
    This antibody recognizes the N-terminus of the dopamine transporter (DAT-NT) in mice and humans.
  • 经测试应用
    适用于: IHC-P, ICC, IHC-Frmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Fusion protein corresponding to Dopamine Transporter. Fusion protein containing the dopamine transporter N-terminal amino acids 1-66.

  • 阳性对照
    • IHC-P: Mouse brain tissue.

性能

应用

Our Abpromise guarantee covers the use of ab5990 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/1000. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
ICC 1/100 - 1/1000.
IHC-Fr 1/500.

靶标

  • 功能
    Amine transporter. Terminates the action of dopamine by its high affinity sodium-dependent reuptake into presynaptic terminals.
  • 疾病相关
    Defects in SLC6A3 are the cause of dystonia-parkinsonism infantile (DYTPRI) [MIM:613135]. It is a neurodegenerative disorder characterized by infantile onset of parkinsonism and dystonia. Other neurologic features include global developmental delay, bradikinesia and pyramidal tract signs.
  • 序列相似性
    Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A3 subfamily.
  • 细胞定位
    Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DA transporter antibody
    • DAT 1 antibody
    • DAT antibody
    • DAT1 antibody
    • Dopamine transporter 1 antibody
    • Dopamine transporter antibody
    • PKDYS antibody
    • SC6A3_HUMAN antibody
    • SLC6A3 antibody
    • Sodium dependent dopamine transporter antibody
    • Sodium-dependent dopamine transporter antibody
    • Solute carrier family 6 (neurotransmitter transporter dopamine), member 3 antibody
    • Solute carrier family 6 (neurotransmitter transporter), member 3 antibody
    • Solute carrier family 6 member 3 antibody
    • Variable number tandem repeat (VNTR) antibody
    see all

图片

  • ab5990 staining of dopamine transporter in mouse brain (substantia nigra) tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Heat-mediated antigen retrieval was carried out using citric acid. Samples were incubated with primary antibody (1/1000) for two hours at room temperature. A Biotin-conjugated goat anti-rat IgG polyclonal was used as the secondary antibody.

文献

This product has been referenced in:
  • Zhou Q  et al. Impairment of PARK14-dependent Ca(2+) signalling is a novel determinant of Parkinson's disease. Nat Commun 7:10332 (2016). ICC ; Mouse . Read more (PubMed: 26755131) »
  • Postupna NO  et al. Flow cytometry analysis of synaptosomes from post-mortem human brain reveals changes specific to Lewy body and Alzheimer's disease. Lab Invest 94:1161-72 (2014). Flow Cyt . Read more (PubMed: 25068655) »

See all 8 Publications for this product

客户评价及客户问答

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Brain
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

提交于 May 16 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Antigen retrieval step
None
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Permeabilization
No
Fixative
Paraformaldehyde
Username

Dr. Karine Thibault

Verified customer

提交于 Aug 19 2013

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (rat dopaminergic neuron)
Specification
rat dopaminergic neuron
Fixative
Formaldehyde
Permeabilization
No
Blocking step
saponin as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.1% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Apr 05 2013

Thank you for contacting us.

ab104224
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I am very pleased to hear that your customer would like to accept our offer and test ab104224 in IHC-Fr. To redeem this offer, plea...

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Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

The details you have kindly provided will enable us to investigate this...

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Thank you for your enquiry,.

We recommend that antibodies should be stable for at least 1 year at the recommended storage temperature. This can be much longer for frozen antibodies. However, please noteour Abpromise guarantee is provided for...

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Thank you for contacting us.  We have three Dopamine Transporter antibodies that will be guaranteed for use in WB on rat samples: ab5981, ab5990, and ab18548.  Please find their datasheets attached.  I would be happy to help you further if you have any...

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Thank you for your enquiry. I regret to inform you that blocking peptides are not available for these 3 antibodies. If you have any additional questions, please don't hesitate to contact us again.

Thank you for your enquiry. Are looking for a dopamine transporter antibody or a dopamine antibody? You indicated ab5990 - Rat monoclonal to Dopamine Transporter - which is suitable for use in ICC but has not yet been tested for cross-reactivity with s...

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I'm sorry to hear you are having a problem with ab5990. Rat DAT contains various carbohydrate moieties and neuramnidase and N-Glycanase treatment reduces it size to ~55kD, so I am wondering if this could be the reason why you see a lower MW band. T...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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