Anti-Dopamine Receptor D3抗体(ab42114)

概述

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab42114 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB 1/1000 - 1/15000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).Can be blocked with Dopamine Receptor D3 peptide (ab128688).
ELISA 1/50000.
Dot blot 1/50000.
IP Use at an assay dependent dilution. 2 µl of this antibody will immunoprecpitate 65-80% dopamine recombinant D3 receptor protein from COS1 cells.

靶标

  • 功能This is one of the five types (D1 to D5) of receptors for dopamine. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase. Promotes cell proliferation.
  • 组织特异性Brain.
  • 疾病相关Genetic variation in DRD3 is associated with essential tremor hereditary type 1 (ETM1) [MIM:190300]. ETM1 is the most common movement disorder. The main feature is postural tremor of the arms. Head, legs, trunk, voice, jaw, and facial muscles also may be involved. The condition can be aggravated by emotions, hunger, fatigue and temperature extremes, and may cause a functional disability or even incapacitation. Inheritance is autosomal dominant.
  • 序列相似性Belongs to the G-protein coupled receptor 1 family.
  • 翻译后修饰Phosphorylated by GRK4 (GRK4-alpha and GRK4-gamma).
  • 细胞定位Cell membrane. Both membrane-bound and scattered in the cytoplasm during basal conditions. Receptor stimulation results in the rapid internalization and sequestration of the receptors at the perinuclear area (5 and 15 minutes), followed by the dispersal of the receptors to the membrane (30 minutes). DRD3 and GRK4 co-localize in lipid rafts of renal proximal tubule cells.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 3dopamine receptor antibody
    • D antibody
    • D(3) dopamine receptor antibody
    • Dopamine D3 receptor antibody
    • Dopamine receptor D3 antibody
    • DRD3 antibody
    • DRD3_HUMAN antibody
    • ETM1 antibody
    • FET1 antibody
    see all

Anti-Dopamine Receptor D3 antibody 图像

  • Anti-Dopamine Receptor D3 antibody (ab42114) at 1/750 dilution + PC-D3R sample

    Predicted band size : 50 kDa
    Observed band size : 50 kDa
  • ICC/IF image of ab42114 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab42114, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Dopamine Receptor D3 antibody (ab42114)参考文献

This product has been referenced in:
  • Ustione A & Piston DW Dopamine synthesis and D3 receptor activation in pancreatic ß-cells regulates insulin secretion and intracellular [Ca(2+)] oscillations. Mol Endocrinol 26:1928-40 (2012). WB ; Mouse . Read more (PubMed: 22918877) »
  • Krishnan B  et al. Dopamine receptor mechanisms mediate corticotropin-releasing factor-induced long-term potentiation in the rat amygdala following cocaine withdrawal. Eur J Neurosci 31:1027-42 (2010). Read more (PubMed: 20377617) »

See all 2 Publications for this product

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Thank you for contacting us.

The catalogue number is ab105625.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

All our antibodies are for research purposes only and not for diagnostic or therapeutic ends. We therefore mainly test our products in in vitro application such as WB and IHC. All four antibodies mentioned have not been tested in vivo however this does...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - other (pancreatic islet lysate, membrane fraction)
Loading amount 18 µg
Specification pancreatic islet lysate, membrane fraction
Gel Running Conditions Reduced Denaturing (10% gel)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Verified customer

提交于 Jan 17 2013

Thank you for your email. I am sorry to hear that you have been experiencing problems with this antibody.

I have read the details you have kindly provided and have following further questions

- What is the problem with these products...

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Finally, it is published, please check the product code ab128688 for blocking peptide.

Many thanks for having patience!

I have forwarded the details to datasheet team. They are now busy in correcting the error and publishing the new datasheet. I will let you know soon, the catalogue number of blocking peptide for ab42114.

I apologize for the delays, we are doi...

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Wehave decidedto publish the datasheet of blocking peptide for ab42114; I will send you the catalogue number soon.

Thank you for contacting us.

We can supply the blocking peptide for AB42114. The price will be £113 for 125ug size. Could you let me know if the customer wishes to but this product, I will then contact the datasheet for publishing this...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"