Anti-Dopamine Receptor D3抗体(ab40655)

概述

  • 产品名称Anti-Dopamine Receptor D3抗体
    参阅全部 Dopamine Receptor D3 一抗
  • 描述
    兔多克隆抗体to Dopamine Receptor D3
  • 特异性D3 (2-10) 100% D3 80% Does not cross-react with other Dopamine Receptors (D1, D2, D4, D5)
  • 经测试应用适用于: ICC/IF, Flow Cyt, WBmore details
  • 种属反应性
    与反应: Rat
    预测可用于: Human
  • 免疫原

    D3 (2-10-Cys11) covalently attached onto a carrier protein.

  • 阳性对照
    • Whole rat brain homogenate This antibody gave a positive result when used in the following methanol fixed cell lines: PC12

性能

  • 形式Liquid
  • 存放说明Store at +4°C short term (1-2 weeks) (add antibacterial agent). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: Whole serum
  • Concentration information loading...
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab40655 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/5000. Stains specific cells in various regions of PLP-fixed rat brain sections including dedritc labelling in the molecular layers as well as the plasma membranes of neurons in layer 5 or cortex and the glomeruli of the olfactory bulb.
Flow Cyt Use at an assay dependent concentration. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
WB 1/500. Detects a band of approximately 50 kDa (predicted molecular weight: 44 kDa).Can be blocked with Dopamine Receptor D3 peptide (ab137997). Blocking with 5% goat or donkey serum significantly reduces background as compared to BSA or milk.

靶标

  • 功能This is one of the five types (D1 to D5) of receptors for dopamine. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase. Promotes cell proliferation.
  • 组织特异性Brain.
  • 疾病相关Genetic variation in DRD3 is associated with essential tremor hereditary type 1 (ETM1) [MIM:190300]. ETM1 is the most common movement disorder. The main feature is postural tremor of the arms. Head, legs, trunk, voice, jaw, and facial muscles also may be involved. The condition can be aggravated by emotions, hunger, fatigue and temperature extremes, and may cause a functional disability or even incapacitation. Inheritance is autosomal dominant.
  • 序列相似性Belongs to the G-protein coupled receptor 1 family.
  • 翻译后修饰Phosphorylated by GRK4 (GRK4-alpha and GRK4-gamma).
  • 细胞定位Cell membrane. Both membrane-bound and scattered in the cytoplasm during basal conditions. Receptor stimulation results in the rapid internalization and sequestration of the receptors at the perinuclear area (5 and 15 minutes), followed by the dispersal of the receptors to the membrane (30 minutes). DRD3 and GRK4 co-localize in lipid rafts of renal proximal tubule cells.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 3dopamine receptor antibody
    • D antibody
    • D(3) dopamine receptor antibody
    • Dopamine D3 receptor antibody
    • Dopamine receptor D3 antibody
    • DRD3 antibody
    • DRD3_HUMAN antibody
    • ETM1 antibody
    • FET1 antibody
    see all

Anti-Dopamine Receptor D3 antibody 图像

  • ab40655 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab40655 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Dopamine Receptor D3 antibody (ab40655)参考文献

ab40655 has not yet been referenced specifically in any publications.

Product Wall

All our antibodies are for research purposes only and not for diagnostic or therapeutic ends. We therefore mainly test our products in in vitro application such as WB and IHC. All four antibodies mentioned have not been tested in vivo however this does...

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Thank you for your patience.

I am happy to confirm that we were able to add the immunogen peptides for the antibody anti-Dopamine receptor 1 (ab40653) and the anti-Dopamine receptor 3 (ab40655).

The immunogen peptide for ab40653 can...

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Thank you for contacting us with your questions, and for yourpatience while I have been in touch with the lab regarding your enquiry.

I have confirmed with the lab that this is taken from the human DRD3 amino acid sequence, which can be found...

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Thank you for your enquiry.

I am sorry to confirm that regrettablythese lot numbers for these antibodies are no longer available. These are very old lot numbers.

Should you have any further questions, please do not hesitate to con...

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Thank you for contacting us.
We currently haven't tested this antibody in immunohistochemistry so we do not know how this antibody behaves in IHC. The DDR3 receptors localizes either in cytoplasm or membrane so the staining should be cytoplasmic o...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Rat Cell (Spleen cells)
Specification Spleen cells
Preparation Cell harvesting/tissue preparation method: Spleen cell suspensions were obtained by gently grinding tissue into RPMI 1640 culture medium (Sigma-Aldrich, Steinheim, Germany) under sterile conditions. Mononuclear cells were separated by Ficoll–Hypaque density gradient (1.083 g/ml) centrifugation. The mononuclear cells concentrated at the surface were collected and washed twice in RPMI 1640 medium. After cell counting, 1 x 10 6 cells/sample were stained with the corresponding antibodies.
Sample buffer: RPMI 1640 culture medium (Sigma-Aldrich, Steinheim, Germany)
Fixation FACSlysing BD
Permeabilization No
Gating Strategy Mononuclear Cells
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提交于 Jun 02 2011

Thank you for your enquiry. The immunogen, ASLSQLSSH, is from the N terminus, as shown in the alignment at the following link: http://www.ebi.ac.uk/cgi-bin/clustalw/result?tool=clustalw&jobid=clustalw-20070926-21493553&poll=yes This is an ext...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"