Anti-Dopamine Receptor D1抗体(ab40653)


  • 产品名称
    Anti-Dopamine Receptor D1抗体
    参阅全部 Dopamine Receptor D1 一抗
  • 描述
    兔多克隆抗体to Dopamine Receptor D1
  • 特异性
    This antibody shows reactivity with: D1 Dopamine Receptor (9-21) 100%; D1 Dopamine Receptor 90%; D2 Dopamine Receptor (272-282) 0%; D2 Dopamine Receptor 0%; D3 Dopamine Receptor (2-10) 0%; D3 Dopamine Receptor 0%; D4 Dopamine Receptor (176-185) 0%; D4 Dopamine Receptor 0%; D5 Dopamine Receptor (23-35) 0%; and D5 Dopamine Receptor 0%.
  • 经测试应用
    适用于: WB, ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Rat, Human
    预测可用于: Mouse, Cow, Pig, Macaque monkey, Rhesus monkey
  • 免疫原

    Synthetic peptide:


    linked to a carrier protein by a carboxyl terminal Cysteine residue linker. Sequence corresponds to amino acids 9-21 of Human Dopamine Receptor D1.

  • 阳性对照
    • ICC: PLP fixed rat brain sections. The antiserum has been found to stain specific cells in various regions, including the Medial septum, Nucleus accumbens, Dentate gyrus, Globus pallidus, Medial forebrain bundle, Cortex regions 1-3, Substantia Nigra reticulata, and the Ventral tegmental area. WB: whole rat brain homogenate.


  • 形式
    Lyophilised:Reconstitute with 0.1ml of PBS which contains 10 mg/ml BSA.
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: None
    Constituents: Whole serum
  • Concentration information loading...
  • 纯度
    Whole antiserum
  • 克隆
  • 同种型
  • 研究领域


Our Abpromise guarantee covers the use of ab40653 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa).Can be blocked with Dopamine Receptor D1 peptide (ab137968). With this antibody, we have found that blocking with 5% goat or donkey serum significantly reduces background as compared to BSA or milk.
ICC/IF 1/5000.
IHC-P 1/500.



  • ab40653 (1:500) staining Dopamine Receptor D1 in human cerebellum using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of cytoplasmic and membrane regions in the fibrous glial cells.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
  • All lanes : Anti-Dopamine Receptor D1 antibody (ab40653) at 1/1000 dilution

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Rat Cortex Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 49 kDa
    Observed band size : 49 kDa
    Additional bands at : 42 kDa,75 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 90 seconds


This product has been referenced in:
  • Wang T  et al. Activation of dopamine D1 receptor decreased NLRP3-mediated inflammation in intracerebral hemorrhage mice. J Neuroinflammation 15:2 (2018). IHC ; Mouse . Read more (PubMed: 29301581) »
  • Zhao Y  et al. Memory retrieval in addiction: a role for miR-105-mediated regulation of D1 receptors in mPFC neurons projecting to the basolateral amygdala. BMC Biol 15:128 (2017). Read more (PubMed: 29282124) »

See all 7 Publications for this product


Western blot

Abcam has not validated the combination of species/application used in this Abreview.
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (4-12% gradient)
Mouse Tissue lysate - whole (hypothalamus and spine)
hypothalamus and spine
Blocking step
normal donkey serum/PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Dr. Tatiana Novoselova

Verified customer

提交于 Jun 17 2013

Thank you for your patience.

I am happy to confirm that we were able to add the immunogen peptides for the antibody anti-Dopamine receptor 1 (ab40653) and the anti-Dopamine receptor 3 (ab40655).

The immunogen peptide for ab40653 can...

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Thank you for your enquiry.

I am sorry to confirm that regrettablythese lot numbers for these antibodies are no longer available. These are very old lot numbers.

Should you have any further questions, please do not hesitate to con...

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Thank you for your inquiry. We would guarantee both ab20066 and ab40653 to work with rat samples in WB in accordance with our Abpromise. I wouldn't be too concerned about the linker ...

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