Anti-Dopamine Receptor D1抗体(ab20066)

概述

  • 产品名称Anti-Dopamine Receptor D1抗体
    参阅全部 Dopamine Receptor D1 一抗
  • 描述
    兔多克隆抗体to Dopamine Receptor D1
  • 特异性Stains specific cells in various regions of PLP fixed Rat brain sections. This includes Medial septum, Nucleus accumbens, Dentate gyrus, Globus pallidus, Medial forebrain bundle, Cortex regions 1-3, Substantia Nigra reticulata, and the Ventral tegmental area. Cross reactivity: D1 Dopamine Receptor (9-21) 100%; D1 Dopamine Receptor 90%; D2 Dopamine Receptor (272-282) 0%; D2 Dopamine Receptor 0%; D3 Dopamine Receptor (2-10) 0%; D3 Dopamine Receptor 0; D4 Dopamine Receptor (176-185) 0%; D4 Dopamine Receptor 0%; D5 Dopamine Receptor (23-35) 0%; D5 Dopamine Receptor 0%
  • 经测试应用适用于: WB, ICC/IF, IHC-FoFr, IHC-P, ELISAmore details
  • 种属反应性
    与反应: Mouse, Rat, Human, Aplysia
  • 免疫原

    Synthetic peptide:

    MDGTGLVVERDFS

    , corresponding to amino acids 9-21 of Human Dopamine Receptor D1

  • 阳性对照
    • Whole Rat brain homogenate.

性能

应用

Our Abpromise guarantee covers the use of ab20066 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 49 kDa).
ICC/IF Use at an assay dependent concentration. PubMed: 19006818
IHC-FoFr Use at an assay dependent concentration.
IHC-P 1/5000.
ELISA Use at an assay dependent concentration.

靶标

Anti-Dopamine Receptor D1 antibody 图像

  • All lanes : Anti-Dopamine Receptor D1 antibody (ab20066) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Rat Forebrain
    Lane 3 : Rat Cortex Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 49 kDa
    Observed band size : 48 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 45 kDa,70 kDa,75 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 90 seconds
  • ab20066 staining Dopamine Receptor D1 in Mouse brain tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with paraformaldehyde and blocked with 5% serum for 60 minutes at 23°C. The sample was incubated with primary antibody (1/600 in PBS + 5% Serum) at 4°C for 13 hours. An undiluted Alexa Fluor®555-conjugated Goat anti-rabbit polyclonal was used as the secondary antibody.

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Anti-Dopamine Receptor D1 antibody (ab20066)参考文献

This product has been referenced in:
  • Kajiwara M  et al. Urinary Dopamine as a Potential Index of the Transport Activity of Multidrug and Toxin Extrusion in the Kidney. Int J Mol Sci 17:N/A (2016). IHC-FoFr ; Mouse . Read more (PubMed: 27483254) »
  • Diaz MR  et al. Moderate Alcohol Exposure during the Rat Equivalent to the Third Trimester of Human Pregnancy Alters Regulation of GABAA Receptor-Mediated Synaptic Transmission by Dopamine in the Basolateral Amygdala. Front Pediatr 2:46 (2014). WB ; Rat . Read more (PubMed: 24904907) »

See all 6 Publications for this product

Product Wall

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Rat Tissue sections (Brain)
Antigen retrieval step None
Permeabilization No
Specification Brain
Blocking step Normal donkey serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 May 11 2015

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5 · Temperature: 20°C
Antigen retrieval step None
Sample Aplysia Tissue sections (ganglia)
Specification ganglia
Permeabilization Yes - triton 0.2%
Fixative Paraformaldehyde
Username

Ms. Francoise Geffroy

Verified customer

提交于 Oct 24 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 20% · Temperature: R°C
Antigen retrieval step None - Buffer/Enzyme Used: citrate buffer pH6
Sample Sheep Tissue sections (Sheep pituitary)
Specification Sheep pituitary
Permeabilization No
Fixative Bouins
Username

Abcam user community

Verified customer

提交于 Oct 10 2014

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step Serum as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 20°C
Sample Monkey Tissue sections (Brain (Frontal Cortex))
Specification Brain (Frontal Cortex)
Permeabilization Yes - 0.5% Triton X-100
Fixative Formaldehyde
Username

Abcam user community

Verified customer

提交于 Apr 18 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Mouse Tissue sections (brain)
Specification brain
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

提交于 Apr 24 2013

Thank you for your reply.

We do have a dopamine receptor D1 protein, ab90384, but I didn't recommend it because we haven't tested it as a positive control in WB.

http://www.abcam.com/Dopamine-Receptor-D1-protein-Active-ab90384.html ...

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Thank you for contacting us. I assume you are refering to the ICC/IF application? If not, please let me know. This application was added based on the publication PubMed: 19006818, and has not been tested in house in this application. The publicat...

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Thank you for your inquiry. We would guarantee both ab20066 and ab40653 to work with rat samples in WB in accordance with our Abpromise. http://www.abcam.com/index.html?pageconfig=resource&rid=10372 I wouldn't be too concerned about the linker ...

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Thank you for your telephone call this afternoon. I can confirm that this Dopamine Receptor D1 antibody (ab20066) has been tested in PLP fixed rat brain sections at 1:5,000 dilution. It stains Medial septum, Nucleus accumbens, Dentate gyrus, Globus ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"