Anti-Dopamine D2 Receptor抗体(ab30743)


  • 产品名称Anti-Dopamine D2 Receptor抗体
    参阅全部 Dopamine D2 Receptor 一抗
  • 描述
    山羊多克隆抗体to Dopamine D2 Receptor
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Dog
  • 免疫原

    Synthetic peptide corresponding to Human Dopamine D2 Receptor aa 250-350 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab32347, ab32348)

  • 阳性对照
    • This antibody gave a positive signal in the following Human adult normal tissue lysates: Brain, Liver, Heart and Skeletal Muscle This antibody gave a negative signal in the following Human adult normal tissue lysates: Testis, Kidney, Colon and Small Intestine




Our Abpromise guarantee covers the use of ab30743 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 51 kDa (predicted molecular weight: 51, 48 kDa).
ICC/IF Use a concentration of 1 µg/ml.


  • 功能This is one of the five types (D1 to D5) of receptors for dopamine. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase.
  • 疾病相关Defects in DRD2 are associated with dystonia type 11 (DYT11) [MIM:159900]; also known as alcohol-responsive dystonia. DYT11 is a myoclonic dystonia. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. DYT11 is characterized by involuntary lightning jerks and dystonic movements and postures alleviated by alcohol. Inheritance is autosomal dominant. The age of onset, pattern of body involvement, presence of myoclonus and response to alcohol are all variable.
  • 序列相似性Belongs to the G-protein coupled receptor 1 family.
  • 细胞定位Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • D(2) dopamine receptor antibody
    • D2 dopamine receptor antibody
    • D2DR antibody
    • D2R antibody
    • Dopamine D2 receptor antibody
    • Dopamine receptor D2 antibody
    • DRD 2 antibody
    • DRD2 antibody
    • DRD2_HUMAN antibody
    see all

Anti-Dopamine D2 Receptor antibody 图像

  • All lanes : Anti-Dopamine D2 Receptor antibody (ab30743) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Human testis tissue lysate - total protein (ab30257)
    Lane 3 : Human liver tissue lysate - total protein (ab29889)
    Lane 4 : Human heart tissue lysate - total protein (ab29431)
    Lane 5 : Human skeletal muscle tissue lysate - total protein (ab29330)
    Lane 6 : Human kidney tissue lysate - total protein (ab30203)
    Lane 7 : Human colon tissue lysate - total protein (ab30051)
    Lane 8 : Human small intestine tissue lysate - total protein (ab29276)

    Lysates/proteins at 10 µg per lane.

    Rabbit polyclonal to Goat IgG (Alexa Fluor® 680) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 51, 48 kDa
    Observed band size : 51 kDa (why is the actual band size different from the predicted?)
  • ICC/IF image of ab30743 stained PC12 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab30743, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96931 Alexa Fluor® 488 donkey anti-goat IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Dopamine D2 Receptor antibody (ab30743)参考文献

ab30743 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us.

The MSDS files have been added to the online datasheets of these 3 antibodies (ab21218, ab32349, ab30743). You can find them now there or below:


Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Mouse Tissue sections (Brain)
Specification Brain
Fixative Paraformaldehyde
Permeabilization No

Dr. Sophie Pezet

Verified customer

提交于 Mar 20 2012