Anti-Dopamine D2 Receptor抗体(ab21218)

概述

  • 产品名称Anti-Dopamine D2 Receptor抗体
    参阅全部 Dopamine D2 Receptor 一抗
  • 描述
    兔多克隆抗体to Dopamine D2 Receptor
  • 特异性Cross reacts 100% with D2 dopamine receptor, 70% with D2S dopamine receptor and 60% with D2L dopamine receptor. Does not cross react with D1, D3, D4 or D5 dopamine receptors. Western immunoblots using whole rat brain homogenate resulted in a pair of bands being detected at ~48 and ~51kDa.
  • 经测试应用适用于: IP, WB, ELISA, ICCmore details
  • 种属反应性
    与反应: Rat, Human
    预测可用于: Mouse, Cow, Dog, Chimpanzee, African Green Monkey
  • 免疫原

    Synthetic peptide:

    AARRAQELEME

    , corresponding to amino acids 272-282 of Human Dopamine D2 Receptor

  • 阳性对照
    • rat brain lysate

性能

应用

Our Abpromise guarantee covers the use of ab21218 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use at an assay dependent concentration. PubMed: 18261457
WB 1/800. Detects a band of approximately 48-51 kDa.

We recommend incubating with the antibody for 1h if the background is high as well as diluting the antibody further. Also blocking with non-bovine products (e.g. goat milk, normal goat serum) is recommended. Use of cow milk or BSA is not preferred.

Dilution optimised using chromogenic detection, and we recommend diluting further when using chemiluminescent substrates.

ELISA Use at an assay dependent concentration.
ICC 1/5000.

靶标

  • 功能This is one of the five types (D1 to D5) of receptors for dopamine. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase.
  • 疾病相关Defects in DRD2 are associated with dystonia type 11 (DYT11) [MIM:159900]; also known as alcohol-responsive dystonia. DYT11 is a myoclonic dystonia. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. DYT11 is characterized by involuntary lightning jerks and dystonic movements and postures alleviated by alcohol. Inheritance is autosomal dominant. The age of onset, pattern of body involvement, presence of myoclonus and response to alcohol are all variable.
  • 序列相似性Belongs to the G-protein coupled receptor 1 family.
  • 细胞定位Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • D(2) dopamine receptor antibody
    • D2 dopamine receptor antibody
    • D2DR antibody
    • D2R antibody
    • Dopamine D2 receptor antibody
    • Dopamine receptor D2 antibody
    • DRD 2 antibody
    • DRD2 antibody
    • DRD2_HUMAN antibody
    see all

Anti-Dopamine D2 Receptor antibody 图像

  • All lanes : Anti-Dopamine D2 Receptor antibody (ab21218) at 1/800 dilution

    Lane 1 : Rat striatum whole tissue lysate
    Lane 2 : Rat striatum whole tissue lysate
    Lane 3 : Rat striatum whole tissue lysate
    Lane 4 : Rat striatum whole tissue lysate
    Lane 5 : Rat striatum whole tissue lysate
    Lane 6 : Rat striatum whole tissue lysate
    Lane 7 : Rat striatum whole tissue lysate
    Lane 8 : Rat striatum whole tissue lysate

    Lysates/proteins at 4 µg per lane.

    Secondary
    HRP conjugated goat anti-rabbit IgG (H+L)
    Developed using the ECL technique

    Performed under non-reducing conditions.

    Observed band size : 50 kDa (why is the actual band size different from the predicted?)


    Exposure time : 10 seconds

    This image is courtesy of an anonymous Abreview

    See Abreview

Anti-Dopamine D2 Receptor antibody (ab21218)参考文献

This product has been referenced in:
  • Zong D  et al. Harnessing the lysosome-dependent antitumor activity of phenothiazines in human small cell lung cancer. Cell Death Dis 5:e1111 (2014). WB ; Human . Read more (PubMed: 24625970) »
  • Chen Y  et al. Distinctive expression and cellular distribution of dopamine receptors in the pancreatic islets of rats. Cell Tissue Res 357:597-606 (2014). Rat . Read more (PubMed: 24825414) »

See all 6 Publications for this product

Product Wall

Thank you for your response.

Currently this product is only offered in this buffer. As the EasyLink kits are only effective with 0.5% BSA, and are only effective with purified antibodies, this product would need to be purified. We do offer kit...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Brain)
Gel Running Conditions Reduced Denaturing (10%)
Loading amount 30 µg
Specification Brain
Blocking step Blotting-grade blocker (Bio-Rad) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Oct 27 2016

That antibody is presented as unpurified whole antiserum, and the IgG concentration is not determined. Total protein in serum is typically 85mg/ml, and BSA has been added to a concentration of 10mg/ml. The antibody concentration in antiserum is typical...

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Application Western blot
Loading amount 50 µg
Gel Running Conditions Non-reduced Non-Denaturing (Native) (stacking gel: 4%, resolving gel: 5-13%)
Sample Rat Tissue lysate - other (hippocampi total membrane fraction)
Specification hippocampi total membrane fraction
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Ms. Sunetra Jitkar

Verified customer

提交于 Feb 19 2014

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Brain)
Permeabilization Yes - triton x-100
Specification Brain
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Oct 29 2013

Application Western blot
Sample Mouse Purified protein (brain lysate)
Loading amount 8 µg
Specification brain lysate
Gel Running Conditions Non-reduced Denaturing (4-15% Tris-HCl)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

提交于 Apr 09 2013

Thank you very much for contacting us with your questions, and for your patience while I've been in touch with the lab regarding this antibody.

The cross-reactivity data was determined by indirect ELISA, so there are no blots showing this dat...

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Thank you for contacting us.

The MSDS files have been added to the online datasheets of these 3 antibodies (ab21218, ab32349, ab30743). You can find them now there or below:

ab21218: http://www.abcam.com/ps/Products/21/ab21218/Msds...

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Thank you for contacting us.

I am very sorry to hear that you have been experiencing troubles when using our products. I would also like to thank you for providing me with details of you protocol.Our products are covered by Abpromise guara...

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Thank you for contacting Abcam.

While we do not offer this product in a conjugated form we do offer a number of direct fluorophore conjugation kits for our antibodies. Our EasyLink direct conjugation kits may be found by following this link: h...

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