Anti-Dopamine beta Hydroxylase抗体(ab96615)

概述

  • 产品名称
    Anti-Dopamine beta Hydroxylase抗体
    参阅全部 Dopamine beta Hydroxylase 一抗
  • 描述
    兔多克隆抗体to Dopamine beta Hydroxylase
  • 经测试应用
    适用于: WB, IHC-P, IHC-Frmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant protein fragment corresponding to a region within amino acids 8 and 290 of Dopamine beta Hydroxylase (NP_000778).

  • 阳性对照
    • WB: SH-SY5Y postive control, 293T negative control IHC-P: SW480 xenograft

性能

应用

Our Abpromise guarantee covers the use of ab96615 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/3000. Predicted molecular weight: 69 kDa.
IHC-P 1/100 - 1/500.
IHC-Fr Use at an assay dependent concentration.

靶标

  • 功能
    Conversion of dopamine to noradrenaline.
  • 通路
    Catecholamine biosynthesis; (R)-noradrenaline biosynthesis; (R)-noradrenaline from dopamine: step 1/1.
  • 疾病相关
    Defects in DBH are the cause of dopamine beta-hydroxylase deficiency (DBH deficiency) [MIM:223360]; also known as norepinephrine deficiency or noradrenaline deficiency. This disorder is characterized by profound deficits in autonomic and cardiovascular function, but apparently only subtle signs, if any, of central nervous system dysfunction.
  • 序列相似性
    Belongs to the copper type II ascorbate-dependent monooxygenase family.
    Contains 1 DOMON domain.
  • 细胞定位
    Cytoplasmic vesicle > secretory vesicle lumen. Cytoplasmic vesicle > secretory vesicle > chromaffin granule lumen and Cytoplasmic vesicle > secretory vesicle membrane. Cytoplasmic vesicle > secretory vesicle > chromaffin granule membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • dbh antibody
    • DBM antibody
    • Dopamine beta hydroxylase antibody
    • Dopamine beta monooxygenase antibody
    • Dopamine beta-hydroxylase (dopamine beta-monooxygenase) antibody
    • Dopamine beta-monooxygenase antibody
    • DOPO_HUMAN antibody
    • OTTHUMP00000022501 antibody
    • Soluble dopamine beta-hydroxylase antibody
    see all

图片

  • All lanes : Anti-Dopamine beta Hydroxylase antibody (ab96615) at 1/2000 dilution

    Lane 1 : SH-SY5Y whole cell extract
    Lane 2 : 293T whole cell extract

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 69 kDa

    Separated by 7.5% SDS-PAGE.

  • mmunohistochemical analysis of Dopamine beta Hydroxylase in paraffin embedded SW480 xenograft, using ab96615 at a 1/500 dilution.
  • ab96615 staining Dopamine beta Hydroxylase in human colorectal tumor tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with formaldehyde, permeabilized with 0.3% Triton X-100 in PBS and blocked with 5% goat serum for 1 hour at 24°C. Samples were incubated with primary antibody (1/1000) for 3 hours at 24°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG monoclonal (1/2000) was used as the secondary antibody.

    See Abreview

文献

This product has been referenced in:
  • Liu Q  et al. Human neural crest stem cells derived from human ESCs and induced pluripotent stem cells: induction, maintenance, and differentiation into functional schwann cells. Stem Cells Transl Med 1:266-78 (2012). ICC/IF ; Human . Read more (PubMed: 23197806) »

See 1 Publication for this product

客户评价及客户问答

Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Sample
Human Tissue sections (Colorectal tumors)
Specification
Colorectal tumors
Permeabilization
Yes - 1xPBS+0.3%t-X-100
Fixative
Formaldehyde
Username

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Verified customer

提交于 Oct 09 2014

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