Anti-Dopamine beta Hydroxylase抗体(ab96615)

概述

  • 产品名称Anti-Dopamine beta Hydroxylase抗体
    参阅全部 Dopamine beta Hydroxylase 一抗
  • 描述
    兔多克隆抗体to Dopamine beta Hydroxylase
  • 经测试应用适用于: WB, IHC-P, ICC/IF, IHC-Frmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant protein fragment corresponding to a region within amino acids 8 and 290 of Dopamine beta Hydroxylase (NP_000778).

  • 阳性对照
    • WB: 293T, A431, H1299, HeLa, HepG2, MOLT4 and Raji cell lysates ICC/IF: HeLa cell IHC-P: SW480 xenograft

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab96615 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/3000. Predicted molecular weight: 69 kDa.
IHC-P 1/100 - 1/500.
ICC/IF 1/100 - 1/200.
IHC-Fr Use at an assay dependent concentration.

靶标

  • 功能Conversion of dopamine to noradrenaline.
  • 通路Catecholamine biosynthesis; (R)-noradrenaline biosynthesis; (R)-noradrenaline from dopamine: step 1/1.
  • 疾病相关Defects in DBH are the cause of dopamine beta-hydroxylase deficiency (DBH deficiency) [MIM:223360]; also known as norepinephrine deficiency or noradrenaline deficiency. This disorder is characterized by profound deficits in autonomic and cardiovascular function, but apparently only subtle signs, if any, of central nervous system dysfunction.
  • 序列相似性Belongs to the copper type II ascorbate-dependent monooxygenase family.
    Contains 1 DOMON domain.
  • 细胞定位Cytoplasmic vesicle > secretory vesicle lumen. Cytoplasmic vesicle > secretory vesicle > chromaffin granule lumen and Cytoplasmic vesicle > secretory vesicle membrane. Cytoplasmic vesicle > secretory vesicle > chromaffin granule membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • dbh antibody
    • DBM antibody
    • Dopamine beta hydroxylase antibody
    • Dopamine beta monooxygenase antibody
    • Dopamine beta-hydroxylase (dopamine beta-monooxygenase) antibody
    • Dopamine beta-monooxygenase antibody
    • DOPO_HUMAN antibody
    • OTTHUMP00000022501 antibody
    • Soluble dopamine beta-hydroxylase antibody
    see all

Anti-Dopamine beta Hydroxylase antibody 图像

  • Anti-Dopamine beta Hydroxylase antibody (ab96615) at 1/5000 dilution + HepG2 whole cell lysate at 30 µg

    Predicted band size : 69 kDa
  • Immunofluorescence analysis of Dopamine beta Hydroxylase in paraformaldehyde fixed HeLa cell, using ab96615 at a 1/200 dilution.
    Lower image: merged with DNA probe.
  • mmunohistochemical analysis of Dopamine beta Hydroxylase in paraffin embedded SW480 xenograft, using ab96615 at a 1/500 dilution.
  • ab96615 staining Dopamine beta Hydroxylase in human colorectal tumor tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with formaldehyde, permeabilized with 0.3% Triton X-100 in PBS and blocked with 5% goat serum for 1 hour at 24°C. Samples were incubated with primary antibody (1/1000) for 3 hours at 24°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG monoclonal (1/2000) was used as the secondary antibody.

    See Abreview

Anti-Dopamine beta Hydroxylase antibody (ab96615)参考文献

This product has been referenced in:
  • Liu Q  et al. Human neural crest stem cells derived from human ESCs and induced pluripotent stem cells: induction, maintenance, and differentiation into functional schwann cells. Stem Cells Transl Med 1:266-78 (2012). ICC/IF ; Human . Read more (PubMed: 23197806) »

See 1 Publication for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Sample Human Tissue sections (Colorectal tumors)
Specification Colorectal tumors
Permeabilization Yes - 1xPBS+0.3%t-X-100
Fixative Formaldehyde
Username

Abcam user community

Verified customer

提交于 Oct 09 2014

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

Read More

Thank you for taking time to complete our questionnaire. I am sorry to hear that this antibody is not providing satisfactory results.

The details provided will enable us to investigate this case and will provide us with vital information for...

Read More

Thank you for contacting us.

I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"