Anti-Dopamine beta Hydroxylase抗体(ab19353)

概述

  • 产品名称
    Anti-Dopamine beta Hydroxylase抗体
    参阅全部 Dopamine beta Hydroxylase 一抗
  • 描述
    羊多克隆抗体to Dopamine beta Hydroxylase
  • 经测试应用
    适用于: ICC, WB, ICC/IF, IHC-P, IHC-Frmore details
  • 种属反应性
    与反应: Mouse, Rat, Cow, Human
  • 免疫原

    Synthetic peptide corresponding to Rat Dopamine beta Hydroxylase aa 43-62.
    Sequence:

    SEPPESPFPYHIPLDPEGTL

  • 常规说明
    Detects human, bovine, rat and mouse DBH. It is not effective for immunohistochemistry in human brain

性能

应用

Our Abpromise guarantee covers the use of ab19353 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC 1/2500.
WB 1/5000. Detects a band of approximately 72 kDa.
ICC/IF 1/5000.
IHC-P 1/2000.
IHC-Fr Use at an assay dependent concentration.

靶标

  • 功能
    Conversion of dopamine to noradrenaline.
  • 通路
    Catecholamine biosynthesis; (R)-noradrenaline biosynthesis; (R)-noradrenaline from dopamine: step 1/1.
  • 疾病相关
    Defects in DBH are the cause of dopamine beta-hydroxylase deficiency (DBH deficiency) [MIM:223360]; also known as norepinephrine deficiency or noradrenaline deficiency. This disorder is characterized by profound deficits in autonomic and cardiovascular function, but apparently only subtle signs, if any, of central nervous system dysfunction.
  • 序列相似性
    Belongs to the copper type II ascorbate-dependent monooxygenase family.
    Contains 1 DOMON domain.
  • 细胞定位
    Cytoplasmic vesicle > secretory vesicle lumen. Cytoplasmic vesicle > secretory vesicle > chromaffin granule lumen and Cytoplasmic vesicle > secretory vesicle membrane. Cytoplasmic vesicle > secretory vesicle > chromaffin granule membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • dbh antibody
    • DBM antibody
    • Dopamine beta hydroxylase antibody
    • Dopamine beta monooxygenase antibody
    • Dopamine beta-hydroxylase (dopamine beta-monooxygenase) antibody
    • Dopamine beta-monooxygenase antibody
    • DOPO_HUMAN antibody
    • OTTHUMP00000022501 antibody
    • Soluble dopamine beta-hydroxylase antibody
    see all

图片

  • Immunofluorescent staining of DBH in rat neuron cells using ab19353.

  • Immunofluorescent stain of DBH in rat brain tissue using ab19353.

文献

This product has been referenced in:
  • Mahata SK  et al. Effect of heart failure on catecholamine granule morphology and storage in chromaffin cells. J Endocrinol 230:309-23 (2016). Read more (PubMed: 27402067) »
  • Talavera-Adame D  et al. Enhancement of embryonic stem cell differentiation promoted by avian chorioallantoic membranes. Tissue Eng Part A 15:3193-200 (2009). Read more (PubMed: 19364272) »

See all 2 Publications for this product

客户评价及客户问答

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry free floating
Sample
Mouse Tissue sections (Mouse brain and spinal cord)
Specification
Mouse brain and spinal cord
Username

Abcam user community

Verified customer

提交于 Feb 22 2017

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry free floating
Sample
Mouse Tissue sections (Brain: Coronal sections of striatum and cortex)
Specification
Brain: Coronal sections of striatum and cortex
Username

Abcam user community

Verified customer

提交于 Jan 28 2016

DISCOUNT CODE: xxx
Expiration date: xxx
Value: xxx

I am very pleased to hear you would like to accept our offer and test ab19353 in IHC-Fr. This code will give you xxx off your next order before the expiration date. To redeem this off...

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Merci beaucoup de votre réponse et de vous avoir pris le temps de remplir le questionnaire.

Effectivement le point qui saute à l'œilen regardant leprotocole, est la méthode d'extraction de la protéine et pr&e...

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Je suis désolée d'apprendre que ab19353 ne vous donne pas de bons résultats en Western blot.

Veuillez trouver ci-jointlequestionnaire discuté au téléphone qui nous permet de regrouper le plus d'inform...

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Thank you for sending your protocol information. We haven't tested this antibody with perfusion-fixed or free floating tissue, but we generally recommend using an antigen retrieval method with formaldehyde-fixed tissue, as formaldehyde crosslinks p...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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