Anti-Dopamine抗体[2B11] (ab8281)

概述

  • 产品名称
    Anti-Dopamine抗体[2B11]
    参阅全部 Dopamine 一抗
  • 描述
    小鼠单克隆抗体[2B11] to Dopamine
  • 特异性
    This antibody detects catecholamine Affinity of this antibody: Kd = 10-8 for DA-BSA Kd = 10-7 for NA-BSA Kd = 10-4 for unconjugated DA
  • 经测试应用
    适用于: ICC, Neutralisingmore details
  • 免疫原

    Chemical/ Small Molecule conjugated to BSA.

  • 常规说明


    Detection of catecholamine-secreting cells.

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 纯度
    IgG fraction
  • Primary antibody说明
    Detection of catecholamine-secreting cells.
  • 克隆
    单克隆
  • 克隆编号
    2B11
  • 骨髓瘤
    Sp2/0
  • 同种型
    IgG1
  • 轻链类型
    unknown
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab8281 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC Use at an assay dependent concentration.
Neutralising Use at an assay dependent concentration.

靶标

  • 相关性
    Dopamine (C6H3OH)2-CH2CH2NH2)is a catecholamine neurotransmitter in the brain. Its chemical name is 4-(2-aminoethyl)benzene-1,2-diol. Dopamine is a hormone released by the hypothalamus. Its main function is to inhibit the release of prolactin from the anterior lobe of the pituitary. It can be used as a sympathomimetic drug, producing effects such as increased heart rate and blood pressure.
  • 细胞定位
    Secreted
  • 别名
    • 3 4 dihydroxyphenethylamine antibody
    • 3 hydroxytyramine antibody
    • DA antibody
    • Dopamine antibody
    • Oxytyramine antibody
    see all

文献

This product has been referenced in:
  • Baldrich E & Muñoz FX Carbon nanotube wiring: a tool for straightforward electrochemical biosensing at magnetic particles. Anal Chem 83:9244-50 (2011). Read more (PubMed: 22040046) »

See 1 Publication for this product

客户评价及客户问答

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Leukemia cells)
Permeabilization
Yes - 0.5% Triton-X100
Specification
Leukemia cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

提交于 Jun 29 2017

Thank you for your enquiry. Yes, since the primary antibody was raised in mouse, this secondary will recognize it. I hope this information helps, please do not hesitate to contact us if you need any more advice or information,

Dopamine conjugated with BSA has been used as the immunogen. However, the antibody is not conjugated.

According to literature data dopamine cross-links to some intracellular component during fixation/permeabilization and it remains inside the cell.

This antibody was raised against human dopamine, cross reactivity with other species has not yet been tested.

1% BSA solutions should be uses as normal as a blocking agent in IHC for both the primary and the secondary antibody. The immunogen was a dopamine-BSA conjugate, BSA faciltates the immune response.

This antibody is a mouse monoclonal (IgG1) to human dopamine, not to the D-2 receptor. As far as we are aware, the antibody has not yet been tested in mouse.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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