Anti-Dopamine抗体[2B11] (ab8281)


  • 产品名称Anti-Dopamine抗体[2B11]
    参阅全部 Dopamine 一抗
  • 描述
    小鼠单克隆抗体[2B11] to Dopamine
  • 特异性This antibody detects catecholamine Affinity of this antibody: Kd = 10-8 for DA-BSA Kd = 10-7 for NA-BSA Kd = 10-4 for unconjugated DA
  • 经测试应用适用于: ICC, Neutralisingmore details
  • 免疫原

    Chemical/ Small Molecule conjugated to BSA.

  • 常规说明

    Detection of catecholamine-secreting cells.


  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 纯度IgG fraction
  • Primary antibody说明Detection of catecholamine-secreting cells.
  • 克隆单克隆
  • 克隆编号2B11
  • 骨髓瘤Sp2/0
  • 同种型IgG1
  • 轻链类型unknown
  • 研究领域


Our Abpromise guarantee covers the use of ab8281 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC Use at an assay dependent concentration.
Neutralising Use at an assay dependent concentration.


  • 相关性Dopamine (C6H3OH)2-CH2CH2NH2)is a catecholamine neurotransmitter in the brain. Its chemical name is 4-(2-aminoethyl)benzene-1,2-diol. Dopamine is a hormone released by the hypothalamus. Its main function is to inhibit the release of prolactin from the anterior lobe of the pituitary. It can be used as a sympathomimetic drug, producing effects such as increased heart rate and blood pressure.
  • 细胞定位Secreted
  • 别名
    • 3 4 dihydroxyphenethylamine antibody
    • 3 hydroxytyramine antibody
    • DA antibody
    • Dopamine antibody
    • Oxytyramine antibody
    see all

Anti-Dopamine antibody [2B11] (ab8281)参考文献

This product has been referenced in:
  • Baldrich E & Muñoz FX Carbon nanotube wiring: a tool for straightforward electrochemical biosensing at magnetic particles. Anal Chem 83:9244-50 (2011). Read more (PubMed: 22040046) »

See 1 Publication for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Leukemia cells)
Permeabilization Yes - 0.5% Triton-X100
Specification Leukemia cells
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative Formaldehyde

Abcam user community

Verified customer

提交于 Jun 29 2017

Thank you for your enquiry. Yes, since the primary antibody was raised in mouse, this secondary will recognize it. I hope this information helps, please do not hesitate to contact us if you need any more advice or information,

Dopamine conjugated with BSA has been used as the immunogen. However, the antibody is not conjugated.

According to literature data dopamine cross-links to some intracellular component during fixation/permeabilization and it remains inside the cell.

This antibody was raised against human dopamine, cross reactivity with other species has not yet been tested.

1% BSA solutions should be uses as normal as a blocking agent in IHC for both the primary and the secondary antibody. The immunogen was a dopamine-BSA conjugate, BSA faciltates the immune response.

This antibody is a mouse monoclonal (IgG1) to human dopamine, not to the D-2 receptor. As far as we are aware, the antibody has not yet been tested in mouse.