Anti-Dopamine抗体[2B11] (ab1001)

概述

  • 产品名称
    Anti-Dopamine抗体[2B11]
    参阅全部 Dopamine 一抗
  • 描述
    小鼠单克隆抗体[2B11] to Dopamine
  • 特异性
    The affinity of this antibody for dopamine-BSA is 10 nM and for noradrenaline-BSA is 100 nM. Affinity for unconjugated catecholamines(dopamine, noradrenaline, adrenaline) is lower (approx. 100 µM). At 1 uM, this antibody inhibits dopamine binding to membrane receptor from human corpus striatum. Cross-reactivity (%): Dopamine = 100% Noradrenaline = <15% Adrenaline = <10% Normetanephrine = 0% 3-methoxy-tyramine = 0% L-DOPA = 0% Homovanillic acid = 0% Vanillic acid = 0% Vanillylmandelic acid = 0% 4-hydroxy-3-methoxyphenyl glycol = 0% 3,4-dihydroxyphenylacetic acid = 0% Tyramine = 0% beta-phenylethylamine = 0% Amphetamine = 0%
  • 经测试应用
    适用于: Blocking, IHC-P, IHC-Frmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length protein corresponding to Dopamine conjugated to Bovine Serum Albumin (BSA). Immunogen = Dopamine conjugated to bovine serum albumin.

  • 常规说明

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by July 2018 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. 

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    pH: 7.40
    Preservative: 0.1% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 纯度
    Protein A purified
  • 克隆
    单克隆
  • 克隆编号
    2B11
  • 同种型
    IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab1001 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Blocking Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

靶标

  • 相关性
    Dopamine (C6H3OH)2-CH2CH2NH2)is a catecholamine neurotransmitter in the brain. Its chemical name is 4-(2-aminoethyl)benzene-1,2-diol. Dopamine is a hormone released by the hypothalamus. Its main function is to inhibit the release of prolactin from the anterior lobe of the pituitary. It can be used as a sympathomimetic drug, producing effects such as increased heart rate and blood pressure.
  • 细胞定位
    Secreted
  • 别名
    • 3 4 dihydroxyphenethylamine antibody
    • 3 hydroxytyramine antibody
    • DA antibody
    • Dopamine antibody
    • Oxytyramine antibody
    see all

Anti-Dopamine antibody [2B11] 图像

  • ab1001 staining human adrenal tissue sections by IHC-P.  Sections were formaldehyde fixed and subjected to heat mediated antigen retrieval in citrate buffer (pH 6) prior to blocking in 1% serum for 30 minutes at 22°C.  The primary antibody was diluted 1/1000 and incubated with the sample for 30 minutes at 22°C.  A HRP conjugated goat anti-mouse antibody diluted 1/400 was used as the secondary.

    See Abreview

Anti-Dopamine antibody [2B11] (ab1001)参考文献

This product has been referenced in:
  • Rowell C  et al. Chemoprevention of breast cancer, proteomic discovery of genistein action in the rat mammary gland. J Nutr 135:2953S-2959S (2005). Read more (PubMed: 16317154) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (adrenal)
Specification
adrenal
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH 6.0
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Oct 05 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (adrenal)
Specification
adrenal
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer pH 6.0
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Oct 05 2007

Thank you for sending this enquiry because it helped us to find a typographical error in our data sheet. The affinity of this antibody for dopamine-BSA should be 10 nM or 10(-8) M (the data sheet says 10 mM), and the affinity for noradrenaline-BSA shou...

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This antibody should work in IHC on paraformaldehyde-fixed tissue without glutaraldehyde. However, we do not have any information on how well this antibody will work on macaca brain sections.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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