Anti-DOPA Decarboxylase/DDC抗体[CL2962] (ab211535)
Key features and details
- Mouse monoclonal [CL2962] to DOPA Decarboxylase/DDC
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG1
概述
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产品名称
Anti-DOPA Decarboxylase/DDC抗体[CL2962]
参阅全部 DOPA Decarboxylase/DDC 一抗 -
描述
小鼠单克隆抗体[CL2962] to DOPA Decarboxylase/DDC -
宿主
Mouse -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Rat, Human
预测可用于: Cow, Pig, Drosophila melanogaster不与反应: Mouse -
免疫原
Recombinant fragment corresponding to Human DOPA Decarboxylase/DDC aa 100-250.
Database link: P20711 -
表位
Binds to an epitope located within the peptide sequence MDWLGKMLEL (aa 119-128) as determined by overlapping synthetic peptides. -
阳性对照
- IHC: Rat substantia nigra and human substantia nigra tissues. WB: Human kidney tissue lysate ICC/IF: Rat substantia nigra tissue
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol, 59% PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
CL2962 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab211535于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/1000. Predicted molecular weight: 53 kDa.
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IHC-P |
1/1000 - 1/2500.
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ICC/IF |
Use at an assay dependent concentration.
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说明 |
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WB
1/500 - 1/1000. Predicted molecular weight: 53 kDa. |
IHC-P
1/1000 - 1/2500. |
ICC/IF
Use at an assay dependent concentration. |
靶标
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功能
Catalyzes the decarboxylation of L-3,4-dihydroxyphenylalanine (DOPA) to dopamine, L-5-hydroxytryptophan to serotonin and L-tryptophan to tryptamine. -
通路
Catecholamine biosynthesis; dopamine biosynthesis; dopamine from L-tyrosine: step 2/2. -
疾病相关
Defects in DDC are the cause of aromatic L-amino-acid decarboxylase deficiency (AADCD) [MIM:608643]. AADCD deficiency is an inborn error in neurotransmitter metabolism that leads to combined serotonin and catecholamine deficiency. It causes developmental and psychomotor delay, poor feeding, lethargy, ptosis, intermittent hypothermia, gastrointestinal disturbances. The onset is early in infancy and inheritance is autosomal recessive. -
序列相似性
Belongs to the group II decarboxylase family. - Information by UniProt
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数据库链接
- Entrez Gene: 280762 Cow
- Entrez Gene: 35190 Drosophila melanogaster
- Entrez Gene: 1644 Human
- Entrez Gene: 24311 Rat
- Omim: 107930 Human
- SwissProt: P27718 Cow
- SwissProt: P05031 Drosophila melanogaster
- SwissProt: P20711 Human
see all -
别名
- AADC antibody
- Aromatic L Amino Acid Decarboxylase antibody
- Aromatic-L-amino-acid decarboxylase antibody
see all
图片
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Anti-DOPA Decarboxylase/DDC antibody [CL2962] (ab211535) at 1/500 dilution + Human kidney tissue lysate
Predicted band size: 53 kDa -
Immunohistochemical analysis of human kidney tissue staining DDC with ab211535 at 1/1000 dilution.
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Immunocytochemistry/Immunofluorescent analysis of rat substantia nigra cells staining DOPA Decarboxylase with ab211535.
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Immunohistochemical analysis of paraffin-embedded human substantia nigra tissue labeling DOPA Decarboxylase/DDC with ab211535 at 1/1000 dilution.
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Immunohistochemical analysis of human fallopian tube tissue staining DDC with ab211535 at 1/1000 dilution.
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Immunohistochemical analysis of paraffin-embedded rat substantia nigra tissue labeling DOPA Decarboxylase/DDC with ab211535 at 1/1000 dilution.
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab211535 被引用在 2 文献中.
- Britto-Júnior J et al. Endothelium-derived dopamine modulates EFS-induced contractions of human umbilical vessels. Pharmacol Res Perspect 8:e00612 (2020). PubMed: 32567793
- Kim H et al. Modeling G2019S-LRRK2 Sporadic Parkinson's Disease in 3D Midbrain Organoids. Stem Cell Reports 12:518-531 (2019). PubMed: 30799274