驴抗羊IgG H&L (HRP) (ab97125)
Key features and details
- Donkey Anti-Sheep IgG H&L (HRP)
- Conjugation: HRP
- Host species: Donkey
- Isotype: IgG
- Suitable for: ICC, IHC-P, ELISA, WB
概述
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产品名称
驴抗羊IgG H&L (HRP)
参阅全部 IgG 二抗 -
宿主
Donkey -
靶标种属
Sheep -
特异性
By immunoelectrophoresis and ELISA this antibody reacts specifically with Sheep IgG and with light chains common to other Sheep immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. -
经测试应用
适用于: ICC, IHC-P, ELISA, WBmore details -
免疫原
Other Immunogen Type corresponding to Sheep IgG.
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偶联物
HRP
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 6.8
Constituents: 0.2% BSA, 0.05% CMIT/MIT based preservative -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP). -
共轭说明
Molar enzyme/ antibody protein ratio is 4:1 -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Substrate reagent
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab97125于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ICC |
Use at an assay dependent dilution.
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|
IHC-P |
1/200 - 1/5000.
|
|
ELISA |
1/10000 - 1/100000. (primary)
|
|
WB | (1) |
1/5000 - 1/50000. Colorimetric: 1/500 - 1/30000, Chemiluminescent: 1/10000 - 1/50000
|
说明 |
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ICC
Use at an assay dependent dilution. |
IHC-P
1/200 - 1/5000. |
ELISA
1/10000 - 1/100000. (primary) |
WB
1/5000 - 1/50000. Colorimetric: 1/500 - 1/30000, Chemiluminescent: 1/10000 - 1/50000 |
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (10)
ab97125 被引用在 10 文献中.
- Manian KV et al. 3D iPSC modeling of the retinal pigment epithelium-choriocapillaris complex identifies factors involved in the pathology of macular degeneration. Cell Stem Cell 28:846-862.e8 (2021). PubMed: 33784497
- Sharrock AV et al. Engineering the Escherichia coli Nitroreductase NfsA to Create a Flexible Enzyme-Prodrug Activation System. Front Pharmacol 12:701456 (2021). PubMed: 34163368
- Ponia SS et al. Mitophagy antagonism by ZIKV reveals Ajuba as a regulator of PINK1 signaling, PKR-dependent inflammation, and viral invasion of tissues. Cell Rep 37:109888 (2021). PubMed: 34706234
- López-Tello J et al. Fetal and trophoblast PI3K p110a have distinct roles in regulating resource supply to the growing fetus in mice. Elife 8:N/A (2019). PubMed: 31241463
- Li Y et al. Endocytic recycling and vesicular transport systems mediate transcytosis of Leptospira interrogans across cell monolayer. Elife 8:N/A (2019). PubMed: 31012847
- Cheng X et al. Morphological and molecular comparisons between tibialis anterior muscle and levator veli palatini muscle: A preliminary study on their augmentation potential. Exp Ther Med 15:247-253 (2018). PubMed: 29375687
- Van de Bittner GC et al. Nasal neuron PET imaging quantifies neuron generation and degeneration. J Clin Invest 127:681-694 (2017). WB . PubMed: 28112682
- Koch F et al. Metabolic Response to Heat Stress in Late-Pregnant and Early Lactation Dairy Cows: Implications to Liver-Muscle Crosstalk. PLoS One 11:e0160912 (2016). WB . PubMed: 27513961
- White CI et al. Cardiomyocyte and Vascular Smooth Muscle-Independent 11ß-Hydroxysteroid Dehydrogenase 1 Amplifies Infarct Expansion, Hypertrophy, and the Development of Heart Failure After Myocardial Infarction in Male Mice. Endocrinology 157:346-57 (2016). WB . PubMed: 26465199
- Suzuki M et al. Activation of systemic, but not local, renin-angiotensin system is associated with upregulation of TNF-a during prolonged fasting in northern elephant seal pups. J Exp Biol 216:3215-21 (2013). PubMed: 23685967