驴Anti-Sheep IgG H&L (DyLight® 650) (ab96942)

概述

  • 产品名称驴Anti-Sheep IgG H&L (DyLight® 650)
    参阅全部 IgG 二抗
  • 描述
    驴多克隆抗体二抗to羊IgG - H&L (DyLight® 650)
  • 靶标种属Sheep
  • 特异性By immunoelectrophoresis and ELISA this antibody reacts specifically with Sheep IgG and with light chains common to other Sheep immunoglobulins. No antibody was detected against non immunoglobulin serum proteins.
  • 经测试应用适用于: Flow Cyt, IHC-P, ICC/IFmore details
  • 偶联物DyLight® 650. Ex: 654nm, Em: 673nm

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 650.
  • 克隆多克隆
  • 同种型IgG
  • 常规说明DyLight® 650 replaces DyLight® 649.
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab96942 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt 1/50 - 1/200.
IHC-P 1/50 - 1/500. DyLight®650 replaces DyLight®649. The DyLight®650 excitation is 652nm and the emission is 672nm. DyLight®650 hence provides the same far-red fluorescence and photostability as the DyLight®649 dye. Please refer to the vial in order to check whether the product you received is DyLight®649 or DyLight®650. This information however will have no impact on your experiments. For more information please refer to www.abcam.com/DyLight650.
ICC/IF 1/50 - 1/500.

Donkey Anti-Sheep IgG H&L (DyLight® 650) 图像

  • ab62116 staining MAP1LC3A in mouse splinocytes by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 1% and blocked with 2% serum for 1 hour at 25°C. Samples were incubated with primary antibody (1 µg/ml in PBS/2% FBS) for 24 hour at 4°C. A DyLight® 650-conjugated donkey anti-sheep IgG H&L (ab96942) (1/1000) was used as the secondary antibody.

    See Abreview

  • Emission spectra of DyLight® fluorochromes available in our catalog.
    Line colors represent the approximate visible colors of the wavelength maxima.

Donkey Anti-Sheep IgG H&L (DyLight® 650) (ab96942)参考文献

ab96942 has not yet been referenced specifically in any publications.

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Merci de votre réponse.

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