驴抗羊IgG H&L (Alkaline Phosphatase) (ab6901)
Key features and details
- Donkey Anti-Sheep IgG H&L (Alkaline Phosphatase)
- Conjugation: Alkaline Phosphatase
- Host species: Donkey
- Suitable for: ICC/IF, IHC-Fr, IHC-P, WB, ELISA, Immunomicroscopy, Dot blot
Related conjugates and formulations
概述
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产品名称
驴抗羊IgG H&L (Alkaline Phosphatase)
参阅全部 IgG 二抗 -
宿主
Donkey -
靶标种属
Sheep -
经测试应用
适用于: ICC/IF, IHC-Fr, IHC-P, WB, ELISA, Immunomicroscopy, Dot blotmore details -
免疫原
Full length native Sheep IgG (purified).
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偶联物
Alkaline Phosphatase
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 0.00136% Zinc chloride, 0.0095% Magnesium chloride, 0.79% Tris HCl, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 1% BSA -
Concentration information loading...
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纯度
Affinity purified -
纯化说明
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities -
共轭说明
Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons) -
克隆
多克隆 -
研究领域
应用
应用 | Ab评论 | 说明 |
---|---|---|
ICC/IF | ||
IHC-Fr | ||
IHC-P | ||
WB | ||
ELISA | ||
Immunomicroscopy | ||
Dot blot |
说明 |
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应用说明
Dot: Use at an assay dependant dilution.
ELISA: 1/3000 - 1/14000.
IHC-P: Use at an assay dependant dilution.
IHC-Fr: Use at an assay dependant dilution.
IM: Use at an assay dependant dilution.
WB: Use at an assay dependant dilution.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ELISA: 1/3000 - 1/14000.
IHC-P: Use at an assay dependant dilution.
IHC-Fr: Use at an assay dependant dilution.
IM: Use at an assay dependant dilution.
WB: Use at an assay dependant dilution.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab6901 被引用在 4 文献中.
- Fernandes B et al. Intensifying Continuous Production of Gag-HA VLPs at High Cell Density Using Stable Insect Cells Adapted to Low Culture Temperature. Front Bioeng Biotechnol 10:917746 (2022). PubMed: 35845394
- Pilipovic K et al. Long-Term Effects of Repetitive Mild Traumatic Injury on the Visual System in Wild-Type and TDP-43 Transgenic Mice. Int J Mol Sci 22:N/A (2021). PubMed: 34205342
- Fernandes B et al. Integrating high cell density cultures with adapted laboratory evolution for improved Gag-HA virus-like particles production in stable insect cell lines. Biotechnol Bioeng 118:2536-2547 (2021). PubMed: 33764532
- Wei Y et al. Mast Cell-Specific Expression of Human Siglec-8 in Conditional Knock-in Mice. Int J Mol Sci 20:N/A (2018). PubMed: 30577572