驴抗大鼠IgG H&L (HRP)预吸附二抗(ab102265)
Key features and details
- Donkey Anti-Rat IgG H&L (HRP) preadsorbed
- Conjugation: HRP
- Host species: Donkey
- Isotype: IgG
- Suitable for: IHC-P, ICC, ELISA, WB
Related conjugates and formulations
概述
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产品名称
驴抗大鼠IgG H&L (HRP)预吸附二抗
参阅全部 IgG 二抗 -
宿主
Donkey -
靶标种属
Rat -
特异性
By immunoelectrophoresis and ELISA this antibody reacts specifically with rat IgG and with light chains common to other rat immunoglobulins. No antibody was detected against nonimmunoglobulin serum proteins. Less than 1% cross reactivity to bovine, human, mouse, rabbit and sheep IgG was detected. This antibody may cross react with IgG from other species.
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经测试应用
适用于: IHC-P, ICC, ELISA, WBmore details -
最小交叉反应
Chicken, Cow, Goat, Horse, Human, Mouse, Rabbit, Sheep more details -
偶联物
HRP
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 6.8
Constituents: 0.2% BSA, 0.05% CMIT/MIT based preservative -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
Antiserum was cross absorbed using bovine, chicken, goat, horse, human, mouse, rabbit and sheep immunosorbents to remove cross reactivity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads. Fragments were conjugated to HRP. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Substrate reagent
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab102265于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
1/200 - 1/5000.
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ICC |
1/200 - 1/5000.
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ELISA |
1/10000 - 1/100000. (Primary).
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WB |
1/5000 - 1/50000. Colorimetric: 1/5000 - 1/30000. Chemiluminescent: 1/10000 - 1/50000.
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说明 |
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IHC-P
1/200 - 1/5000. |
ICC
1/200 - 1/5000. |
ELISA
1/10000 - 1/100000. (Primary). |
WB
1/5000 - 1/50000. Colorimetric: 1/5000 - 1/30000. Chemiluminescent: 1/10000 - 1/50000. |
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab102265 被引用在 5 文献中.
- Jbara A et al. RBFOX2 modulates a metastatic signature of alternative splicing in pancreatic cancer. Nature 617:147-153 (2023). PubMed: 36949200
- Sangkhae V et al. Erythroferrone contributes to iron mobilization for embryo erythropoiesis in iron-deficient mouse pregnancies. Am J Hematol 97:1348-1358 (2022). PubMed: 36071577
- Taha A et al. Omega-3 and Omega-6 polyunsaturated fatty acids stimulate vascular differentiation of mouse embryonic stem cells. J Cell Physiol 235:7094-7106 (2020). PubMed: 32020589
- Bischoff FC et al. Identification and Functional Characterization of Hypoxia-Induced Endoplasmic Reticulum Stress Regulating lncRNA (HypERlnc) in Pericytes. Circ Res 121:368-375 (2017). PubMed: 28611075
- Müller N et al. B Lymphocyte Stimulator (BLyS) Is Expressed in Human Adipocytes In Vivo and Is Related to Obesity but Not to Insulin Resistance. PLoS One 9:e94282 (2014). WB ; Human . PubMed: 24728308