驴抗大鼠IgG H&L (DyLight® 550)预吸附二抗(ab102261)
Key features and details
- Donkey Anti-Rat IgG H&L (DyLight® 550) preadsorbed
- Conjugation: DyLight® 550. Ex: 562nm, Em: 576nm
- Host species: Donkey
- Isotype: IgG
- Suitable for: Flow Cyt, ICC, IHC-P
Related conjugates and formulations
概述
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产品名称
驴抗大鼠IgG H&L (DyLight® 550)预吸附二抗
参阅全部 IgG 二抗 -
宿主
Donkey -
靶标种属
Rat -
特异性
By immunoelectrophoresis and ELISA this antibody reacts specifically with rat IgG and with light chains common to other rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to bovine, chicken, goat, horse, human, mouse, rabbit and sheep IgG was detected. This antibody may cross react with IgG from other species. -
经测试应用
适用于: Flow Cyt, ICC, IHC-Pmore details -
最小交叉反应
Chicken, Cow, Goat, Horse, Human, Mouse, Rabbit, Sheep more details -
偶联物
DyLight® 550. Ex: 562nm, Em: 576nm
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.2% BSA, PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
Antiserum was cross adsorbed using bovine, chicken, goat, horse, human, mouse, rabbit and sheep immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 550. -
克隆
多克隆 -
同种型
IgG -
常规说明
DyLight® 550 replaces DyLight® 549. -
研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab102261于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt |
1/50 - 1/200.
|
|
ICC |
1/50 - 1/500.
|
|
IHC-P |
1/50 - 1/500.
DyLight® 550 replaces DyLight® 549. The DyLight® 550 excitation is 562nm and the emission is 576nm. DyLight® 550 provides the same orange-to-red fluorescence and photostability as the DyLight® 549 dye. Please refer to the vial in order to check whether the product you received is DyLight® 549 or DyLight® 550. This information however will have no impact on your experiments. For more information please refer to DyLight® 550. |
说明 |
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Flow Cyt
1/50 - 1/200. |
ICC
1/50 - 1/500. |
IHC-P
1/50 - 1/500. DyLight® 550 replaces DyLight® 549. The DyLight® 550 excitation is 562nm and the emission is 576nm. DyLight® 550 provides the same orange-to-red fluorescence and photostability as the DyLight® 549 dye. Please refer to the vial in order to check whether the product you received is DyLight® 549 or DyLight® 550. This information however will have no impact on your experiments. For more information please refer to DyLight® 550. |
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (5)
ab102261 被引用在 5 文献中.
- Ike S et al. Cryopreserved allogenic fibroblast sheets: development of a promising treatment for refractory skin ulcers. Am J Transl Res 14:3879-3892 (2022). PubMed: 35836896
- Newman AAC et al. Multiple cell types contribute to the atherosclerotic lesion fibrous cap by PDGFRβ and bioenergetic mechanisms. Nat Metab 3:166-181 (2021). PubMed: 33619382
- Hartmann F et al. SMC-Derived Hyaluronan Modulates Vascular SMC Phenotype in Murine Atherosclerosis. Circ Res 129:992-1005 (2021). PubMed: 34615369
- Hess DL et al. Perivascular cell-specific knockout of the stem cell pluripotency gene Oct4 inhibits angiogenesis. Nat Commun 10:967 (2019). PubMed: 30814500
- Durgin BG et al. Smooth muscle cell-specific deletion of Col15a1 unexpectedly leads to impaired development of advanced atherosclerotic lesions. Am J Physiol Heart Circ Physiol 312:H943-H958 (2017). PubMed: 28283548