驴Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97085)

概述

  • 产品名称驴Anti-Rabbit IgG H&L (HRP) preadsorbed
    参阅全部 IgG 二抗
  • 描述
    驴多克隆抗体二抗to兔IgG - H&L (HRP), pre-adsorbed
  • 靶标种属Rabbit
  • 特异性By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Less than 0.1% cross reactivity to bovine, chicken, goat, horse, human, mouse, pig and rat was detected.
  • 经测试应用适用于: ICC, IHC-P, WB, ELISAmore details
  • 最小交叉反应
    Chicken, Cow, Goat, Human, Mouse, Pig, Rat more details
  • 偶联物HRP

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C.
  • 存储溶液Preservative: 0.1% Proclin
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明Antiserum was cross adsorbed using bovine, chicken, goat, human, mouse, pig and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab97085 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC Use at an assay dependent dilution.
IHC-P 1/200 - 1/5000.
WB 1/5000 - 1/50000. (Colorimetric: 1/5000 - 1/30000; Chemiluminescent: 1/10000 - 1/50000)
ELISA 1/10000 - 1/100000. (Primary ELISA)

Donkey Anti-Rabbit IgG H&L (HRP) preadsorbed 图像

  • ab134047 staining VCAM1 in mouse aorta tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with methanol and blocked with 10% normal donkey serum for 2 hours at room temperature. Samples were incubated with primary antibody (1/200 in PBS + 0.1% Triton + 2% normal donkey serum) for 12 hours at 4°C. A HRP-conjugated donkey anti-rabbit IgG H&L (ab97085) (1/1000) was used as the secondary antibody

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Donkey Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97085)参考文献

ab97085 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"