驴Anti-Rabbit IgG H&L (HRP) preadsorbed (ab7083)

Submit an Abreview Q&A (3)Specific References (2)
Customer reviews and FAQs

概述

  • 产品名称驴Anti-Rabbit IgG H&L (HRP) preadsorbed
    参阅全部 IgG 二抗
  • 靶标种属Rabbit
  • 经测试应用适用于: ICC/IF, IHC-P, WB, ELISA, Immunomicroscopy, Dot blot, IHC-Frmore details
  • 最小交叉反应
    Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rat, Sheep more details
  • 免疫原

    Full length native Rabbit IgG (purified).

  • 偶联物HRP

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C.
  • 存储溶液Preservative: 0.01% Gentamicin Sulfate
    Constituents: 10mg/ml BSA, 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • 纯度IgG fraction
  • 纯化说明This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads
  • 共轭说明Horseradish Peroxidase (HRP)
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab7083 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/1000 - 1/3000.
IHC-P Use at an assay dependent dilution.
WB 1/1000 - 1/3000.
ELISA 1/7500 - 1/30000.
Immunomicroscopy Use at an assay dependent dilution.
Dot blot Use at an assay dependent dilution.
IHC-Fr Use at an assay dependent dilution.

Donkey Anti-Rabbit IgG H&L (HRP) preadsorbed (ab7083)参考文献

This product has been referenced in:
  • Faulkner S  et al. A comparison of the bovine uterine and plasma proteome using iTRAQ proteomics. Proteomics 12:2014-23 (2012). Read more (PubMed: 22623423) »
  • Kashino SS  et al. Initiation of acquired immunity in the lungs of mice lacking lymph nodes after infection with aerosolized Mycobacterium tuberculosis. Am J Pathol 176:198-204 (2010). IHC-P . Read more (PubMed: 20008132) »

See all 2 Publications for this product

发表研究结果有使用 ab7083？请让我们知道，以便我们可以引用本数据表中的参考文章。

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3 Abreviews or Q&A

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Again, thank you so much for the insight. I did the experiment using both adult rat hippocampus and 3-week-old pup liver against B-actin as control. Apparently, both proteins showed a weak bands, the former was expressed at around ˜35kDa and the latter...

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Thank you for contacting us again and sending this new information.

The WB image looks very convincing and proofs that the antibody is actually picking up its target as it should.

Regarding the double band in the pup sample it may be ...

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I truly appreciate your immediate reply especially at this time. From your suggestion, I will try to do a positive control run using a female adult hippocampus sample. Will that be okay? Regarding my inquiry, could you enlighten me on the expression at...

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Thank you for your reply.

Unfortunately, it is quite hard to get some information if this protein expressed in the rat hippocampus. However, I checked the SwissProt site for more information regardingthis for the human protein, and it looks li...

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I recently ordered Anti-Folate binding protein primary antibody (EPR4708) Cat # ab125030 and donkey anti-rabbit IgG H&L (HRP) secondary anitbody Cat # ab7083 to measure the expression of FBP1 (Folate receptor alpha) that has a predicted band ...

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Thank you for resending the image. Based on your description of the upper band as being ˜ 25kD it seems this could be the correct band, as the size can vary a few kD just based on the gel, buffer, etc. Adding a negative control could help ensure that t...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"