概述

  • 产品名称驴Anti-Rabbit IgG H&L (HRP)
    参阅全部 IgG 二抗
  • 靶标种属Rabbit
  • 经测试应用适用于: WB, ELISA, Immunomicroscopy, Dot blot, ICC/IF, IHC-P, IHC-Frmore details
  • 免疫原

    Rabbit IgG, whole molecule

  • 偶联物HRP

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C.
  • 存储溶液Preservative: 0.01% Gentamicin Sulfate
    Constituents: 10mg/ml BSA, 0.15M Sodium chloride, 0.02M Potassium phosphate, pH 7.2
  • Concentration information loading...
  • 纯度IgG fraction
  • 纯化说明Horseradish Peroxidase (HRP).
  • 共轭说明This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab6802 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/3000.
ELISA 1/160000.
Immunomicroscopy Use at an assay dependent concentration.
Dot blot Use at an assay dependent concentration.
ICC/IF 1/500 - 1/2000.
IHC-P 1/500 - 1/2000.
IHC-Fr 1/500 - 1/2000.

Donkey Anti-Rabbit IgG H&L (HRP) 图像

  • IHC image of beta actin staining in human colon formalin fixed paraffin embedded tissue section*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab8227, 5µg/ml overnight at +4°C. An HRP-conjugated secondary (ab6802, 1/2000 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.

    The inset negative control image is secondary-only at 1/500 dilution.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre


  • Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 1 minute

    This image is courtesy of an anonymous Abreview.

    See Abreview


  • Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 1 minute

    This image is courtesy of an Abreview submitted by Min young Kim

    See Abreview

Donkey Anti-Rabbit IgG H&L (HRP) (ab6802)参考文献

This product has been referenced in:

See all 15 Publications for this product

Product Wall

It is normal that the solution is not completely clear. We recommend centrifuging the solution if it is still an unclear suspension after standing at room temperature (and gently mixed with some finger tapping).

Thank you for contacting us.

we tested catalog ab126709in IP, IHC, ICC, and Flow cytometry and it is tested negative. For this reason, we only recommending the product for western blot.

I hope this information is helpful to you. Pleas...

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The ab22696 could be used at 1/10 - 1/100 dilution.
ab54615 at 5-10ug/ml
ab103724 at 5-10ug/ml
ab126709 at 1/500-1/1000 dilution.

ab6820 and ab6802 at 1/500-1/2000 dilution.

I hope thi...

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Thank you for contacting us.

The secondary antibodies you mentioned might work for dot blots with the rabbit polyclonal Histone primaries, as they are guaranteed to work in Western blot which is quite similar to dot blot. However, we have not...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"