驴抗兔IgG H&L (HRP) (ab16284)
Key features and details
- Donkey Anti-Rabbit IgG H&L (HRP)
- Conjugation: HRP
- Host species: Donkey
- Isotype: IgG
- Suitable for: WB
概述
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产品名称
驴抗兔IgG H&L (HRP)
参阅全部 IgG 二抗 -
宿主
Donkey -
靶标种属
Rabbit -
特异性
Reacts with the heavy and light chains of the immunoglobulin molecule. -
经测试应用
适用于: WBmore details -
免疫原
Synthetic peptide corresponding to IgG.
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偶联物
HRP
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading...
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纯度
Protein G purified -
克隆
多克隆 -
同种型
IgG -
常规说明
Part of the AbExcel range. -
研究领域
相关产品
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Substrate reagent
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab16284于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/2000 - 1/20000. Optimized for ECL applications.
|
说明 |
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WB
1/2000 - 1/20000. Optimized for ECL applications. |
图片
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All lanes : Anti-beta Actin antibody - Loading Control (ab8227) at 1 µg/ml
Lanes 1-6 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.
Secondary
Lanes 1 - 2 : Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab16284) at 1/2000 dilution
Lanes 3 - 4 : Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab16284) at 1/10000 dilution
Lanes 5 - 6 : Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab16284) at 1/20000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time : 150 seconds
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (66)
ab16284 被引用在 66 文献中.
- Thejer BM et al. Sigma-2 Receptor Ligand Binding Modulates Association between TSPO and TMEM97. Int J Mol Sci 24:N/A (2023). PubMed: 37047353
- Papandreou ME et al. Nucleophagy delays aging and preserves germline immortality. Nat Aging 3:34-46 (2023). PubMed: 37118512
- Shi Y et al. The Antiviral Factor SERINC5 Impairs the Expression of Non-Self-DNA. Viruses 15:N/A (2023). PubMed: 37766367
- Ruivo CF et al. Extracellular Vesicles from Pancreatic Cancer Stem Cells Lead an Intratumor Communication Network (EVNet) to fuel tumour progression. Gut N/A:N/A (2022). PubMed: 35012996
- Niu F et al. The m6A reader YTHDF2 is a negative regulator for dendrite development and maintenance of retinal ganglion cells. Elife 11:N/A (2022). PubMed: 35179492