驴Anti-Rabbit IgG H&L (HRP) (ab16284)

概述

  • 产品名称
    驴Anti-Rabbit IgG H&L (HRP)
    参阅全部 IgG 二抗
  • 靶标种属
    Rabbit
  • 特异性
    Reacts with the heavy and light chains of the immunoglobulin molecule.
  • 经测试应用
    适用于: WBmore details
  • 免疫原

    Synthetic peptide corresponding to IgG.

  • 偶联物
    HRP

性能

应用

Our Abpromise guarantee covers the use of ab16284 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/20000. Optimized for ECL applications.

图片

  • All lanes : Anti-beta Actin antibody - Loading Control (ab8227) at 1 µg/ml

    Lanes 1-6 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lysates/proteins at 10 µg per lane.

    Secondary
    Lanes 1 - 2 : Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab16284) at 1/2000 dilution
    Lanes 3 - 4 : Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab16284) at 1/10000 dilution
    Lanes 5 - 6 : Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab16284) at 1/20000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Exposure time : 150 seconds
  • Ab16284 was used at dilution 1/5000 with the primary antibody ab52903 in ELISA. See the review on ab52903.
  • Ab16284 was used at dilution 1/2000 with the primary antibody ab28379 in WB. See the review on ab28379.

文献

This product has been referenced in:
  • Cruz IN  et al. Proteomics Analysis of Ovarian Cancer Cell Lines and Tissues Reveals Drug Resistance-associated Proteins. Cancer Genomics Proteomics 14:35-51 (2017). Read more (PubMed: 28031236) »
  • Yang L  et al. AMP-activated protein kinase mediates the effects of lipoprotein-associated phospholipase A2 on endothelial dysfunction in atherosclerosis. Exp Ther Med 13:1622-1629 (2017). Read more (PubMed: 28413519) »

See all 24 Publications for this product

客户评价及客户问答

We have issued a free of charge replacement.

Thank you for your reply. I recommend the following HRP conjugated secondary antibodies to use with these rabbit Anti-GAD65+GAD67 primary antibodies. I have included one product from a donkey host and another from goat. Both are guaranteed and the c...

Read More

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacements for one vial of ab63913 and one vial of ab16284 with the order number 1147505.
...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册