驴Anti-Rabbit IgG H&L (DyLight® 650) preadsorbed (ab96922)

概述

  • 产品名称驴Anti-Rabbit IgG H&L (DyLight® 650) preadsorbed
    参阅全部 IgG 二抗
  • 描述
    驴多克隆抗体二抗to兔IgG - H&L (DyLight® 650), pre-adsorbed
  • 靶标种属Rabbit
  • 特异性By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Less than 0.1% cross reactivity to bovine, chicken, goat, horse, human, mouse, pig and rat was detected.
  • 经测试应用适用于: ICC/IF, IHC-P, Flow Cytmore details
  • 最小交叉反应
    Chicken, Cow, Goat, Horse, Human, Mouse, Pig, Rat more details
  • 偶联物DyLight® 650. Ex: 654nm, Em: 673nm

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明Antiserum was cross adsorbed using bovine, chicken, goat, human, mouse, pig and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 650.
  • 克隆多克隆
  • 同种型IgG
  • 常规说明DyLight® 650 replaces DyLight® 649.
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab96922 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/50 - 1/500.
IHC-P 1/50 - 1/500. DyLight®650 replaces DyLight®649. The DyLight®650 excitation is 652nm and the emission is 672nm. DyLight®650 hence provides the same far-red fluorescence and photostability as the DyLight®649 dye. Please refer to the vial in order to check whether the product you received is DyLight®649 or DyLight®650. This information however will have no impact on your experiments. For more information please refer to www.abcam.com/DyLight650.
Flow Cyt 1/50 - 1/200.

Donkey Anti-Rabbit IgG H&L (DyLight® 650) preadsorbed 图像

  • Emission spectra of DyLight® fluorochromes available in our catalog.
    Line colors represent the approximate visible colors of the wavelength maxima.

Donkey Anti-Rabbit IgG H&L (DyLight® 650) preadsorbed (ab96922)参考文献

This product has been referenced in:
  • Gunness P  et al. 3D organotypic cultures of human HepaRG cells: a tool for in vitro toxicity studies. Toxicol Sci 133:67-78 (2013). Read more (PubMed: 23377618) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"