驴Anti-Rabbit IgG H&L (DyLight® 650) preadsorbed (ab96922)

概述

  • 产品名称
    驴Anti-Rabbit IgG H&L (DyLight® 650) preadsorbed
    参阅全部 IgG 二抗
  • 描述
    驴多克隆抗体二抗to兔IgG - H&L (DyLight® 650), pre-adsorbed
  • 靶标种属
    Rabbit
  • 特异性
    By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Less than 0.1% cross reactivity to bovine, chicken, goat, horse, human, mouse, pig and rat was detected.
  • 经测试应用
    适用于: ICC/IF, IHC-P, Flow Cytmore details
  • 最小交叉反应

    Chicken, Cow, Goat, Horse, Human, Mouse, Pig, Rat more details
  • 偶联物
    DyLight® 650. Ex: 654nm, Em: 673nm

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C.
  • 存储溶液
    Preservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    Antiserum was cross adsorbed using bovine, chicken, goat, human, mouse, pig and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 650.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 常规说明
    DyLight® 650 replaces DyLight® 649.
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab96922 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/50 - 1/500.
IHC-P 1/50 - 1/500. DyLight®650 replaces DyLight®649. The DyLight®650 excitation is 652nm and the emission is 672nm. DyLight®650 hence provides the same far-red fluorescence and photostability as the DyLight®649 dye. Please refer to the vial in order to check whether the product you received is DyLight®649 or DyLight®650. This information however will have no impact on your experiments. For more information please refer to www.abcam.com/DyLight650.
Flow Cyt 1/50 - 1/200.

图片

  • Emission spectra of DyLight® fluorochromes available in our catalog.
    Line colors represent the approximate visible colors of the wavelength maxima.

文献

This product has been referenced in:
  • Spencer KC  et al. Characterization of Mechanically Matched Hydrogel Coatings to Improve the Biocompatibility of Neural Implants. Sci Rep 7:1952 (2017). IHC . Read more (PubMed: 28512291) »
  • Lee SJ  et al. Adult stem cells from the hyaluronic acid-rich node and duct system differentiate into neuronal cells and repair brain injury. Stem Cells Dev 23:2831-40 (2014). IHC (PFA fixed) . Read more (PubMed: 25027245) »

See all 4 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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