驴Anti-Rabbit IgG H&L (DyLight® 550) preadsorbed (ab96920)

概述

  • 产品名称驴Anti-Rabbit IgG H&L (DyLight® 550) preadsorbed
    参阅全部 IgG 二抗
  • 描述
    驴多克隆抗体二抗to兔IgG - H&L (DyLight® 550), pre-adsorbed
  • 靶标种属Rabbit
  • 特异性By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Less than 0.1% cross reactivity to bovine, chicken, goat, horse, human, mouse, pig and rat was detected.
  • 经测试应用适用于: IHC-P, ICC/IF, Flow Cytmore details
  • 最小交叉反应
    Chicken, Cow, Goat, Horse, Human, Mouse, Pig, Rat more details
  • 偶联物DyLight® 550. Ex: 562nm, Em: 576nm

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明Antiserum was cross adsorbed using bovine, chicken, goat, human, mouse, pig and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 550.
  • 克隆多克隆
  • 同种型IgG
  • 常规说明DyLight® 550 replaces DyLight® 549.
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab96920 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/50 - 1/500. DyLight®550 replaces DyLight®549. The DyLight®550 excitation is 562nm and the emission is 576nm. DyLight®550 provides the same orange-to-red fluorescence and photostability as the DyLight®549 dye. Please refer to the vial in order to check whether the product you received is DyLight®549 or DyLight®550. This information however will have no impact on your experiments. For more information please refer to www.abcam.com/DyLight550.
ICC/IF 1/50 - 1/500.
Flow Cyt 1/50 - 1/200.

Donkey Anti-Rabbit IgG H&L (DyLight® 550) preadsorbed 图像

  • Emission spectra of DyLight® fluorochromes available in our catalog.
    Line colors represent the approximate visible colors of the wavelength maxima.

Donkey Anti-Rabbit IgG H&L (DyLight® 550) preadsorbed (ab96920)参考文献

ab96920 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your inquiry.

I strongly suggest to use a pre-absorbed secondary antibody so that the cross-binding to the two rat monoclonals can be minimized.

Since you are already using fluorescence in the green and red spectrum,...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"