驴Anti-Rabbit IgG H&L (DyLight® 488) (ab96891)

概述

  • 产品名称驴Anti-Rabbit IgG H&L (DyLight® 488)
    参阅全部 IgG 二抗
  • 靶标种属Rabbit
  • 特异性By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.
  • 经测试应用适用于: IHC-P, ICC/IF, Flow Cytmore details
  • 偶联物DyLight® 488. Ex: 493nm, Em: 518nm

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 488.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab96891 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/50 - 1/500.
ICC/IF 1/50 - 1/500.
Flow Cyt 1/50 - 1/200.

Donkey Anti-Rabbit IgG H&L (DyLight® 488) 图像

  • Emission spectra of DyLight® fluorochromes available in our catalog.
    Line colors represent the approximate visible colors of the wavelength maxima.

Donkey Anti-Rabbit IgG H&L (DyLight® 488) (ab96891)参考文献

This product has been referenced in:
  • Sakar Y  et al. Impact of high-fat feeding on basic helix-loop-helix transcription factors controlling enteroendocrine cell differentiation. Int J Obes (Lond) N/A:N/A (2014). Read more (PubMed: 24480860) »
  • Ferron PJ  et al. Comparative analysis of the cytotoxic effects of okadaic acid-group toxins on human intestinal cell lines. Mar Drugs 12:4616-34 (2014). ICC/IF . Read more (PubMed: 25196936) »

See all 2 Publications for this product

Product Wall

Thank you for your reply.

I googled for flourocene filter, but could not find an entry. Do you maybe mean fluorescence filter?

What wavelength does the filter cover? I assume it would be in the green range(about 480 - 570 nm) since...

Read More

Thank you for your email.

To answer your questions and make some comments/suggestions that hopefully will help you further:

1) The antibodies (shipped as is) should be stored aliquoted and stored at -20 or -80 degree. This would als...

Read More

Thank you for your email.
I am sorry to hear the antigen retrieval step did not improve your results.

I have looked throught your protocol you sent me and have the following suggestions, comments and questions:

1) You are makin...

Read More

Thank you for your reply.
I'm glad to hear that the secondary antibody shows fluorescence.

As for selecting another secondary antibody, could you please let me know which fluorophore or wavelength range is covered by your miscroscope? Ba...

Read More

Thank you for contacting us.
I am sorry to hear that the antibodie might not be working as expected.

As we discussed over the phone the following troubleshooting tips might help in finding the cause of the problem:

1) Try antig...

Read More

It has been a pleasure to help. We are here until 8:00pm eastern time so not quite as late as the university crowd. Please feel free to contact me if you have any questions.

Thank you for contacting Abcam. We have a large number of secondary antibodies with fluorescent tags. Theeasiest way to find the one that best fits your needs is from the Abcam home page. The secondary antibodies drop down, located in the yellow banner...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"