驴Anti-Rabbit IgG H&L (Biotin) preadsorbed (ab7082)

概述

  • 产品名称驴Anti-Rabbit IgG H&L (Biotin) preadsorbed
    参阅全部 IgG 二抗
  • 靶标种属Rabbit
  • 经测试应用适用于: ICC/IF, WB, ELISA, Immunomicroscopy, Dot blot, IHC-P, IHC-Frmore details
  • 最小交叉反应
    Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rat, Sheep more details
  • 免疫原

    Full length native Rabbit IgG (purified).

  • 偶联物Biotin

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.01% Sodium Azide
    Constituents: 10mg/ml BSA, 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • 纯度IgG fraction
  • 纯化说明Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Goat IgG molecule
  • 共轭说明This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab7082 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF
WB
ELISA
Immunomicroscopy
Dot blot
IHC-P
IHC-Fr
  • 应用说明Dot: Use at an assay dependant dilution.
    ELISA: 1/50000 - 1/200000.
    IHC-P: Use at an assay dependant dilution.
    IHC-Fr: Use at an assay dependant dilution.
    IM: Use at an assay dependant dilution.
    WB: Use at an assay dependant dilution.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Donkey Anti-Rabbit IgG H&L (Biotin) preadsorbed 图像

    • Ab7082 was used at dilution 1/200 with the primary antibody ab77512 in IHC-Fr. See the review on ab77512.

    Donkey Anti-Rabbit IgG H&L (Biotin) preadsorbed (ab7082)参考文献

    This product has been referenced in:
    • Vasistha NA  et al. Cortical and Clonal Contribution of Tbr2 Expressing Progenitors in the Developing Mouse Brain. Cereb Cortex N/A:N/A (2014). IHC (PFA fixed) . Read more (PubMed: 24927931) »

    See 1 Publication for this product

    Product Wall

    I have processed your request to receive ab106843 as a replacement for ab16801. I have also sent ab97055, goat polyclonal to rat (Biotin) aswell. The ab106843 is backordered until December 12th though and so your order will ship as soon as it comes in...

    Read More

    Thank you for your reply. I would be happy to send you ab106843 as a replacement. The secondary antibody that you originally ordered was an anti-rabbit, not an anti-mouse, is that correct? I would also be happy to send you ab97055 as well. ...

    Read More

    Thank you for your reply. The antibody ab55075 has not been proven to work on mouse tissue, there is a chance that the antibody will work on mouse tissue but we cannot guarantee it. The only other antibody in our catalogue that will work on mouse tissu...

    Read More

    I am sorry that you are having problems using ab16801 in IHC-P and in western blot. Normally I would try and offer protocol tips for the IHC-P staining but the fact that you are not getting a signal when using the antibody in western blot does show tha...

    Read More

    The antibodies should be stable at room temperature for that short period of time. It at all possible though, if you can even keep them with an ice pack that would be better. If there is anything else I can help you with, please let me know.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"