AbExcel

驴Anti-Rabbit IgG H&L (Alkaline Phosphatase) (ab97061)

概述

  • 产品名称驴Anti-Rabbit IgG H&L (Alkaline Phosphatase)
    参阅全部 IgG 二抗
  • 靶标种属Rabbit
  • 特异性By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.
  • 经测试应用适用于: ICC, ELISA, WB, IHC-Pmore details
  • 偶联物Alkaline Phosphatase

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, 0.1M Sodium chloride, 50mM HEPES, 1mM Magnesium chloride, 0.1mM Zinc chloride, pH 7.1
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Alkaline Phosphatase (AP).
  • 克隆多克隆
  • 同种型IgG
  • 常规说明Part of the AbExcel range.
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab97061 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC Use at an assay dependent dilution.
ELISA 1/5000 - 1/50000.
WB 1/5000 - 1/50000.
IHC-P 1/100 - 1/1000.

Donkey Anti-Rabbit IgG H&L (Alkaline Phosphatase) 图像

  • All lanes : Anti-beta Actin antibody - Loading Control (ab8227) at 1 µg/ml

    Lanes 1-6 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lysates/proteins at 10 µg per lane.

    Secondary
    Lanes 1 - 2 : Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (Alkaline Phosphatase) (ab97061) at 1/5000 dilution
    Lanes 3 - 4 : Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (Alkaline Phosphatase) (ab97061) at 1/20000 dilution
    Lanes 5 - 6 : Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (Alkaline Phosphatase) (ab97061) at 1/50000 dilution

    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 3 minutes

Donkey Anti-Rabbit IgG H&L (Alkaline Phosphatase) (ab97061)参考文献

This product has been referenced in:
  • Kilinc S  et al. Lysine-specific demethylase-1 (LSD1) is compartmentalized at nuclear chromocenters in early post-mitotic cells of the olfactory sensory neuronal lineage. Mol Cell Neurosci 74:58-70 (2016). Read more (PubMed: 26947098) »
  • Mehrbod P  et al. Simvastatin modulates cellular components in influenza A virus-infected cells. Int J Mol Med 34:61-73 (2014). WB . Read more (PubMed: 24788303) »

See all 2 Publications for this product

Product Wall

Thank you for contacting us.

The link to our indirect ELISA protocol is:
http://www.abcam.com/index.html?pageconfig=resource&rid=11389

I have also attached the protocol as pdf-file.

I hope this information is h...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"