驴Anti-Mouse IgG H&L (Texas Red ®) preadsorbed (ab7059)

概述

  • 产品名称驴Anti-Mouse IgG H&L (Texas Red ®) preadsorbed
    参阅全部 IgG 二抗
  • 靶标种属Mouse
  • 经测试应用适用于: Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr, ELISA, ICC/IFmore details
  • 最小交叉反应
    Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Rabbit, Rat, Sheep more details
  • 免疫原

    Full length native mouse IgG (purified).

  • 偶联物Texas Red ®. Ex: 596nm, Em: 620nm

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.01% Sodium Azide
    Constituents: 10mg/ml BSA, 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • 纯度IgG fraction
  • 纯化说明This product was prepared from monospecific antiserum by immunoaffinity chromatography using mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • 共轭说明Texas RedTM Sulfonyl Chloride (Molecular Weight 625 daltons) Absorption Wavelength: 596 nm Emission Wavelength: 620 nm Fluorochrome/Protein Ratio: 4.9 moles Texas Redä per mole of Rabbit IgG
  • 克隆多克隆
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab7059 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Immunomicroscopy Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent dilution.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
ELISA 1/10000 - 1/50000.
ICC/IF 1/1000 - 1/5000. PubMed: 18263878

Donkey Anti-Mouse IgG H&L (Texas Red ®) preadsorbed (ab7059)参考文献

This product has been referenced in:
  • Planutis K  et al. A novel signaling pathway regulates colon cancer angiogenesis through Norrin. Sci Rep 4:5630 (2014). IHC-P ; Human . Read more (PubMed: 25005225) »
  • Hejna J  et al. Tip60 Is Required for DNA Interstrand Cross-link Repair in the Fanconi Anemia Pathway. J Biol Chem 283:9844-51 (2008). ICC/IF . Read more (PubMed: 18263878) »

See all 2 Publications for this product

Product Wall

Thank you for contacting us.
I am sorry to hear that you received so far no staining with the antibodies.

As we discussed by phone, I'd suggest trying the following:

1) try another antigen retrieval step (e.g. Tris/EDTA, pH 8) ...

Read More

This antibody will detect IgG1 kappa.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"