驴Anti-Mouse IgG H&L (Phycoerythrin) preadsorbed (ab7003)

概述

  • 产品名称驴Anti-Mouse IgG H&L (Phycoerythrin) preadsorbed
    参阅全部 IgG 二抗
  • 靶标种属Mouse
  • 经测试应用适用于: IHC-P, IHC-Fr, Immunomicroscopy, Flow Cyt, ICC/IFmore details
  • 最小交叉反应
    Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Rabbit, Rat, Sheep more details
  • 免疫原

    Mouse IgG whole molecule

  • 偶联物Phycoerythrin. Ex: 488nm, Em: 575nm

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C.
  • 存储溶液Preservative: 0.01% Sodium Azide
    Constituents: 10mg/ml BSA, 0.15M Sodium chloride, 0.02M Potassium phosphate, pH 7.2
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明Prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation
  • 共轭说明R-Phycoerythrin (R-PE) Molecular Weight 240,000 daltons) Absorption Wavelength: 488 nm Emission Wavelength: 575 nm OD566nm/OD280nm: 2.8
  • 克隆多克隆
  • 同种型IgG
  • 常规说明


    Can be used in immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

  • 研究领域

应用

Our Abpromise guarantee covers the use of ab7003 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent dilution.
IHC-Fr Use at an assay dependent dilution.
Immunomicroscopy Use at an assay dependent dilution.
Flow Cyt 1/100 - 1/250.
ICC/IF 1/100 - 1/250.

Donkey Anti-Mouse IgG H&L (Phycoerythrin) preadsorbed 图像

  • Ab7003 was used with the primary antibody ab33315 in Flow Cyt. See Abreview on ab33315.

Donkey Anti-Mouse IgG H&L (Phycoerythrin) preadsorbed (ab7003)参考文献

This product has been referenced in:
  • Marion-Poll L  et al. Fluorescence-activated sorting of fixed nuclei: a general method for studying nuclei from specific cell populations that preserves post-translational modifications. Eur J Neurosci 39:1234-44 (2014). Flow Cyt ; Mouse . Read more (PubMed: 24713002) »

See 1 Publication for this product

Product Wall

I’m sorry for the delay in getting back to you. I have discussed the problem the customer had been encountering with my colleague and as I mentioned the results they have been observing are what would be expected. The Alexa488 is a much stronger fluoro...

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Thank you for contacting us and sorry for the delay in getting back to you.
I have looked at the protocol your customer has been using and I would say that the results they have been observing are what would be expected. The Alexa488 is a much str...

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Thank you for contacting us.  Our F(ab')2 fragment secondary antibodies ab7002 and ab7003 will not contain the Fc region, and this will be covered under our Abpromise guarantee.  If any Fc reactivity is observed in your experiments, we would be happy t...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"