驴Anti-Mouse IgG H&L (DyLight® 550) (ab96876)

概述

  • 产品名称
    驴Anti-Mouse IgG H&L (DyLight® 550)
    参阅全部 IgG 二抗
  • 靶标种属
    Mouse
  • 特异性
    By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG and with light chains common to other Mouse immunoglobulins. No antibody was detected against non immunoglobulin serum proteins.
  • 经测试应用
    适用于: IHC-P, ICC/IF, Flow Cyt, WBmore details
  • 偶联物
    DyLight® 550. Ex: 562nm, Em: 576nm

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C.
  • 存储溶液
    Preservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 550.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 常规说明
    DyLight® 550 replaces DyLight® 549.
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab96876 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/50 - 1/500. DyLight® 550 replaces DyLight®549. The DyLight® 550 excitation is 562nm and the emission is 576nm. DyLight®550 provides the same orange-to-red fluorescence and photostability as the DyLight®549 dye. Please refer to the vial in order to check whether the product you received is DyLight®549 or DyLight®550 – this information however will have no impact on your experiments. For more information please refer to www.abcam.com/DyLight550.
ICC/IF 1/50 - 1/500.
Flow Cyt 1/50 - 1/200.
WB Use at an assay dependent concentration.

Donkey Anti-Mouse IgG H&L (DyLight® 550) 图像

  • Emission spectra of DyLight® fluorochromes available in our catalog.
    Line colors represent the approximate visible colors of the wavelength maxima.
  • Courtesy of an anonymous Abreview

Donkey Anti-Mouse IgG H&L (DyLight® 550) (ab96876)参考文献

This product has been referenced in:
  • de la Mare JA  et al. Extracellular Hsp90 and TGFß regulate adhesion, migration and anchorage independent growth in a paired colon cancer cell line model. BMC Cancer 17:202 (2017). Read more (PubMed: 28302086) »
  • Ferron PJ  et al. Comparative analysis of the cytotoxic effects of okadaic acid-group toxins on human intestinal cell lines. Mar Drugs 12:4616-34 (2014). ICC/IF . Read more (PubMed: 25196936) »

See all 2 Publications for this product

Product Wall

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ab96876 is a Donkey polyclonal anti mouse IgG secondary, and should therefore detect ab13579TRF2 antibody [4A794]which is a mouse IgG1 primary.

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