驴抗小鼠IgG H&L (DyLight® 550) (ab96876)
Key features and details
- Donkey Anti-Mouse IgG H&L (DyLight® 550)
- Conjugation: DyLight® 550. Ex: 562nm, Em: 576nm
- Host species: Donkey
- Isotype: IgG
- Suitable for: IHC-P, ICC/IF, Flow Cyt, WB
Related conjugates and formulations
概述
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产品名称
驴抗小鼠IgG H&L (DyLight® 550)
参阅全部 IgG 二抗 -
宿主
Donkey -
靶标种属
Mouse -
特异性
By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG and with light chains common to other Mouse immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. -
经测试应用
适用于: IHC-P, ICC/IF, Flow Cyt, WBmore details -
偶联物
DyLight® 550. Ex: 562nm, Em: 576nm
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.2% BSA, PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 550. -
克隆
多克隆 -
同种型
IgG -
常规说明
DyLight® 550 replaces DyLight® 549. -
研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab96876于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
1/50 - 1/500.
DyLight® 550 replaces DyLight® 549. The DyLight® 550 excitation is 562nm and the emission is 576nm. DyLight® 550 provides the same orange-to-red fluorescence and photostability as the DyLight® 549 dye. Please refer to the vial in order to check whether the product you received is DyLight® 549 or DyLight® 550 – this information however will have no impact on your experiments. For more information please refer to DyLight® 550. |
|
ICC/IF |
1/50 - 1/500.
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Flow Cyt |
1/50 - 1/200.
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WB |
Use at an assay dependent concentration.
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说明 |
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IHC-P
1/50 - 1/500. DyLight® 550 replaces DyLight® 549. The DyLight® 550 excitation is 562nm and the emission is 576nm. DyLight® 550 provides the same orange-to-red fluorescence and photostability as the DyLight® 549 dye. Please refer to the vial in order to check whether the product you received is DyLight® 549 or DyLight® 550 – this information however will have no impact on your experiments. For more information please refer to DyLight® 550. |
ICC/IF
1/50 - 1/500. |
Flow Cyt
1/50 - 1/200. |
WB
Use at an assay dependent concentration. |
图片
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Formalin-fixed paraffin-embedded sections of human ovarian carcinoma stained for p53 using a mouse anti-p53 (DO-1) antibody, followed by ab96876 at a 1:100 dilution (5 µg/ml). Counterstained with DAPI (blue).
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Emission spectra of DyLight® fluorochromes available in our catalog.
Line colors represent the approximate visible colors of the wavelength maxima. -
All lanes : Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab9484) at 1/1000 dilution
- Lane 1 : Rat Hippocampus
- Lane 2 : Rat Hippocampus
- Lane 3 : Rat Hippocampus
Lysates/proteins at 25 µg per lane.
Secondary:
- Lane 1 : Rabbit polyclonal Secondary Antibody to Mouse IgG - H&L (DyLight® 550), pre-adsorbed (ab98786) at 1/5000 dilution
- Lane 2 : Rabbit polyclonal Secondary Antibody to Mouse IgG - H&L (DyLight® 550), pre-adsorbed (ab98786) at 1/1000 dilution
- Lane 3 : Other Commercial Dye at 1/1000 dilution
Performed under reducing conditions.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (13)
ab96876 被引用在 13 文献中.
- Bos R et al. Altered action potential waveform and shorter axonal initial segment in hiPSC-derived motor neurons with mutations in VRK1. Neurobiol Dis 164:105609 (2022). PubMed: 34990802
- Haydn T et al. Next-generation sequencing reveals a novel role of lysine-specific demethylase 1 in adhesion of rhabdomyosarcoma cells. Int J Cancer 146:3435-3449 (2020). PubMed: 31755110
- Zhang I et al. Nanotherapeutic Modulation of Human Neural Cells and Glioblastoma in Organoids and Monocultures. Cells 9:N/A (2020). PubMed: 33171886
- El-Bazzal L et al. Loss of Cajal bodies in motor neurons from patients with novel mutations in VRK1. Hum Mol Genet 28:2378-2394 (2019). PubMed: 31090908
- Kinslechner K et al. Loss of SR-BI Down-Regulates MITF and Suppresses Extracellular Vesicle Release in Human Melanoma. Int J Mol Sci 20:N/A (2019). PubMed: 30823658