驴抗小鼠IgG H&L (DyLight® 488)预吸附二抗(ab98794)
Key features and details
- Donkey Anti-Mouse IgG H&L (DyLight® 488) preadsorbed
- Conjugation: DyLight® 488. Ex: 493nm, Em: 518nm
- Host species: Donkey
- Isotype: IgG
- Suitable for: Flow Cyt, ICC/IF, IHC-P, WB
概述
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产品名称
驴抗小鼠IgG H&L (DyLight® 488)预吸附二抗
参阅全部 IgG 二抗 -
宿主
Donkey -
靶标种属
Mouse -
经测试应用
适用于: Flow Cyt, ICC/IF, IHC-P, WBmore details -
最小交叉反应
Chicken, Cow, Goat, Horse, Human, Rabbit, Rat, Sheep more details -
偶联物
DyLight® 488. Ex: 493nm, Em: 518nm
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: PBS, 0.2% BSA -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 488. -
克隆
多克隆 -
同种型
IgG -
研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab98794于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Flow Cyt |
1/50 - 1/200.
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|
ICC/IF |
1/40 - 1/500.
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|
IHC-P |
1/40 - 1/400.
|
|
WB |
1/1000 - 1/20000. Predicted molecular weight: 36 kDa.
5% non-fat dry milk in PBST or TBST is recommended for blocking and incubation of antibodies. BSA is not recommended. |
说明 |
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Flow Cyt
1/50 - 1/200. |
ICC/IF
1/40 - 1/500. |
IHC-P
1/40 - 1/400. |
WB
1/1000 - 1/20000. Predicted molecular weight: 36 kDa. 5% non-fat dry milk in PBST or TBST is recommended for blocking and incubation of antibodies. BSA is not recommended. |
图片
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All lanes : Cocktail of mouse anti-hnRNP, rabit anti-actin and goat anti-GAPDH at 1 µg
Lane 1 : HeLa whole cell lysate at 30 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Secondary
All lanes : Cocktail containing ab98794 donkey anti-mouse Dylight 488 (blue); donkey anti-rabbit Dylight 680 (green) and donkey anti-goat Dylight 800 (red) at 0.5 µg
Predicted band size: 36 kDaAcquisition: Syngene G:Box, 34 seconds (blue), 20 seconds (green), and 50 seconds (red)
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Immunohistochemistry immunofluorescent analysis of human ovarian carcinoma tissue using primary mouse anti-p53 at 1/100 dilution and secondary ab98794 at 1/50 with different lots on left and right panels. Counterstain was DAPI.
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Emission spectra of DyLight® fluorochromes available in our catalog.
Line colors represent the approximate visible colors of the wavelength maxima.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (5)
ab98794 被引用在 5 文献中.
- Otte K et al. Eltanexor Effectively Reduces Viability of Glioblastoma and Glioblastoma Stem-Like Cells at Nano-Molar Concentrations and Sensitizes to Radiotherapy and Temozolomide. Biomedicines 10:N/A (2022). PubMed: 36140245
- Hinteregger B et al. Metabolic, Phenotypic, and Neuropathological Characterization of the Tg4-42 Mouse Model for Alzheimer's Disease. J Alzheimers Dis 80:1151-1168 (2021). PubMed: 33646155
- Zhao K et al. Inhibition of Carbonic Anhydrase 2 Overcomes Temozolomide Resistance in Glioblastoma Cells. Int J Mol Sci 23:N/A (2021). PubMed: 35008590
- Shir A et al. S101, an Inhibitor of Proliferating T Cells, Rescues Mice From Superantigen-Induced Shock. J Infect Dis 217:288-297 (2018). PubMed: 29149330
- Gunness P et al. 3D organotypic cultures of human HepaRG cells: a tool for in vitro toxicity studies. Toxicol Sci 133:67-78 (2013). PubMed: 23377618