驴Anti-Mouse IgG H&L (12nm Gold) preadsorbed (ab105277)

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概述

  • 产品名称
    驴Anti-Mouse IgG H&L (12nm Gold) preadsorbed
    参阅全部 IgG 二抗
  • 靶标种属
    Mouse
  • 特异性
    Recognizes the heavy chains on mouse IgG and the light chains common to most murine immunoglobulins. Does not crossreact with non-immunoglobulin serum proteins. Exhibits minimal cross reactivity to Bovine, chicken, goat, guinea pig, hamster, horse, human, rabbit and sheep serum proteins.
  • 经测试应用
    适用于: ELISA, WB, Electron Microscopymore details
  • 最小交叉反应

    Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Rabbit, Sheep more details
  • 偶联物
    Gold 12nm

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C.
  • 存储溶液
    Preservative: 0.05% Sodium Azide
    Constituents: 15mg/ml BSA, 0.15M Sodium chloride, 10mM Sodium phosphate, 10mM Sodium borate, pH 8.5
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    Purified by affinity chromatography.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab105277 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.
WB 1/20 - 1/40.
Electron Microscopy 1/20 - 1/40.

Donkey Anti-Mouse IgG H&L (12nm Gold) preadsorbed (ab105277)参考文献

This product has been referenced in:
  • Rose NF  et al. In vitro evolution of high-titer, virus-like vesicles containing a single structural protein. Proc Natl Acad Sci U S A 111:16866-71 (2014). Electron Microscopy . Read more (PubMed: 25385608) »

See 1 Publication for this product

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What is the recommended dilution for EM and concentration of the product?

For the colloidal gold complexes, we don’t have a measure of the antibody concentration after complexing. We express the product concentration in terms of OD520 = 2.0, which is a measure of the concentration of gold particles. The OD520 is adjust...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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