驴Anti-Goat IgG H&L (Phycoerythrin) preadsorbed (ab7004)

概述

  • 产品名称驴Anti-Goat IgG H&L (Phycoerythrin) preadsorbed
    参阅全部 IgG 二抗
  • 靶标种属Goat
  • 经测试应用适用于: ICC/IF, IHC-P, IHC-Fr, Immunomicroscopy, Flow Cytmore details
  • 最小交叉反应
    Chicken, Guinea pig, Hamster, Horse, Human, Mouse, Rabbit, Rat more details
  • 免疫原

    Goat IgG whole molecule

  • 偶联物Phycoerythrin. Ex: 488nm, Em: 575nm

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C.
  • 存储溶液pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明Prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation
  • 共轭说明R-Phycoerythrin (R-PE) Molecular Weight 240,000 daltons) Absorption Wavelength: 488 nm Emission Wavelength: 575 nm OD566nm/OD280nm: 2.8
  • 克隆多克隆
  • 同种型IgG
  • 常规说明


    Can be used in immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

  • 研究领域

应用

Our Abpromise guarantee covers the use of ab7004 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/100 - 1/250.
IHC-P Use at an assay dependent dilution.
IHC-Fr Use at an assay dependent dilution.
Immunomicroscopy Use at an assay dependent dilution.
As a general guideline, dilutions of 1/50 to 1/200 should be suitable for most applications.
Flow Cyt 1/100 - 1/250. The maximum amount of reagent required to stain 1 x 106 cells in flow cytometry is approximately 1.0µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining.

Donkey Anti-Goat IgG H&L (Phycoerythrin) preadsorbed (ab7004)参考文献

This product has been referenced in:
  • Williams JW  et al. Transcription factor IRF4 drives dendritic cells to promote Th2 differentiation. Nat Commun 4:2990 (2013). Read more (PubMed: 24356538) »
  • Miles A  et al. CCL25 and CCL28 promote alpha4 beta7-integrin-dependent adhesion of lymphocytes to MAdCAM-1 under shear flow. Am J Physiol Gastrointest Liver Physiol 294:G1257-67 (2008). Read more (PubMed: 18308860) »

See all 2 Publications for this product

Product Wall

Application Flow Cytometry
Very bright secondary antibody and PE is one of the best if you have a weak antigen. I used this in a perfect pairing with FITC in a FACS experiment to check pluripotency markers in human embryonic stem cells.
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Verified customer

提交于 Sep 28 2015

Thank you for your enquiry. No reaction was observed with ab7004 against ant-Pepsin, anti-Donkey IgG F(c), Chicken, Guinea Pig, Syrian Hamster, Horse, Human, Mouse, Rabbit, or Rat serum proteins or other goat heavy or light chain proteins. Pig was not ...

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Thank you for your reply. Generally, if you intend to detect an intracellular protein via FACS analysis, you need to permeabilise the cells (i.e. using permeabilization buffer containing saponin) before the staining procedure. This secondary an...

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Thank you for your enquiry and your interest in our products. According to the datasheet, this secondary antibody (ab7004) has been tested for FACS/Flow cytometry. For details on general FACS analysis, please click on the “Protocols“ section on ...

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We do not have a concentration for the antibody as such. However, the concentration of the label is 0.5 mg/ml, and we would estimate the antibody to be about 1 mg/ml.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"