概述

  • 产品名称驴Anti-Goat IgG H&L (HRP)
    参阅全部 IgG 二抗
  • 靶标种属Goat
  • 经测试应用适用于: Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WB, ICC/IFmore details
  • 免疫原

    Full length native Goat IgG (purified).

  • 偶联物HRP

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C.
  • 存储溶液Preservative: 0.01% Gentamicin Sulfate
    Constituents: 10mg/ml BSA, 0.15M Sodium chloride, 0.02M Potassium phosphate, pH 7.2
  • Concentration information loading...
  • 纯度IgG fraction
  • 纯化说明This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • 共轭说明Horseradish Peroxidase (HRP)
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab6885 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Dot blot Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
Immunomicroscopy Use at an assay dependent concentration.
WB Use at an assay dependent concentration.
ICC/IF 1/1000 - 1/2500.

Donkey Anti-Goat IgG H&L (HRP) 图像

  • IHC image of beta Actin staining in Human normal colon formalin fixed paraffin embedded tissue section*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab8229,10ug/ml overnight at +4°C. An HRP-conjugated secondary (Ab6885, 1/250 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.

    The inset negative control image is taken from an identical assay without primary antibody.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Ab6885 was used at dilution 1/5000 with the primary antibody ab36146 in WB. See the review on ab36146.

Donkey Anti-Goat IgG H&L (HRP) (ab6885)参考文献

This product has been referenced in:
  • Faroni A  et al. Purinergic signaling mediated by P2X7 receptors controls myelination in sciatic nerves. J Neurosci Res 92:1259-69 (2014). Read more (PubMed: 24903685) »
  • Boraldi F  et al. Ectopic calcification in ß-thalassemia patients is associated with increased oxidative stress and lower MGP carboxylation. Biochim Biophys Acta 1832:2077-2084 (2013). Read more (PubMed: 23899606) »

See all 14 Publications for this product

Product Wall

Thank you for contacting us.

As I presume these antibodies will be used in ICC/IF (which is the application ab13627 has been tested in), I would then suggest using ab6885 or ab6741 as secondary antibody.

Both should recognise and be...

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I have requested that you be sent 3 new antibodies for the customer and I have also asked that the vials be checked for brown precipitate before being shipped.

Thank you for all your help with this matter and I hope it all works out.
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I am really sorry that the customer is so upset, that was never my intention. I understand that they are well versed in the WB field; howeverI was only trying to get some more information on the protocol that they had used.

If the customers a...

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Thank you for contacting Abcam.

I am sorry that your customer is still having problems with these antibodies. As I mentioned in my previous email in November, could you provide me with the protocol that they are using and I can see if there i...

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I am sorry that you are experiencing problems with the HRP tagged antibodies that you purchased from us. As you mentioned in the email that was sent, it is common for hrp tagged secondaries to have a brown precipitate in the vial, but vortexing should ...

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We recommend the follwing dilutions: ELISA 1:4,000 - 1:10,000 Immunoblotting 1:2,000 - 1:5,000 Immunohistochemistry 1:200 - 1:2,000

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"