驴Anti-Goat IgG H&L (Cy3 ®) preadsorbed (ab6949)

概述

  • 产品名称
    驴Anti-Goat IgG H&L (Cy3 ®) preadsorbed
    参阅全部 IgG 二抗
  • 描述
    驴多克隆抗体二抗to山羊IgG - H&L (Cy3 ®), pre-adsorbed
  • 靶标种属
    Goat
  • 经测试应用
    适用于: ICC/IF, IHC-P, IHC-Fr, ICC, Flow Cyt, ELISAmore details
  • 最小交叉反应

    Cow, Guinea pig, Hamster, Horse, Mouse, Rabbit, Rat more details
  • 免疫原

    Full length native Goat IgG (purified).

  • 偶联物
    Cy3 ®. Ex: 552nm, Em: 565nm

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C.
  • 存储溶液
    Preservative: 0.01% Sodium Azide
    Constituents: 10mg/ml BSA, 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above.
  • 共轭说明
    Cy3.29 (Cyanine 3.29-OSu) (Molecular Weight 949 daltons) Absorption Wavelength: 552 nm Emission Wavelength: 565 nm Fluorochrome/Protein Ratio: 8.1 moles Cy3 per mole of Donkey IgG
  • 克隆
    多克隆
  • 同种型
    IgG
  • 常规说明
    This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited. This material is also subject to proprietary rights of GE Healthcare Bio-Sciences Corp. and Carnegie Mellon University and made and sold under License from GE Healthcare Bio-Sciences Corp. This product is licensed for sale only for research. It is NOT licensed for any other use. There is no implied license hereunder for any commercial use. COMMERCIAL USE shall include: 1 Sale, lease, license or other transfer of the material or any material derived or produced from it. 2 Sale, lease, license or other grant of rights to use this material or any material derived or produced from it. 3 Use of this material to perform services for a fee for third parties. If you require a commercial license to use this material and do not have one, please return this material, unopened to Abcam Plc of 330 Cambridge Science Park, Cambridge, CB4 0FL, and any money paid for the material will be refunded.


    This secondary antibody is specifically designed for the detection of multiple primary antibodies (polyclonal or monoclonal) of different host species in experiments where cells are simultaneously labeled without unwanted cross reaction.

  • 研究领域

应用

Our Abpromise guarantee covers the use of ab6949 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/1000 - 1/5000.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent dilution.
ELISA 1/10000 - 1/50000.

文献

This product has been referenced in:
  • Shi J  et al. Adipose-Derived Stem Cells Cocultured with Chondrocytes Promote the Proliferation of Chondrocytes. Stem Cells Int 2017:1709582 (2017). Read more (PubMed: 28133485) »
  • Liu L  et al. Noise induced hearing loss impairs spatial learning/memory and hippocampal neurogenesis in mice. Sci Rep 6:20374 (2016). Read more (PubMed: 26842803) »

See all 10 Publications for this product

客户评价及客户问答

Abreviews
Application
Immunohistochemistry (PFA fixed)
I used this secondary antibody on PFA-fixed frozen brain sections to bind to the primary antibody sox 2 (goat).
Username

Abcam user community

Verified customer

提交于 Dec 08 2014

I'm not sure why you would want to use this secondary antibody conjugated to a fluorophore in Western Blotting. The secondary would bind to your goat primary, but you would not easily be able to detect it. I would definitely not recommend your using th...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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