驴抗山羊IgG H&L (Cy2 ®)预吸附二抗(ab6948)
Key features and details
- Donkey Anti-Goat IgG H&L (Cy2 ®) preadsorbed
- Conjugation: Cy2 ®. Ex: 489nm, Em: 505nm
- Host species: Donkey
- Suitable for: ICC/IF, IHC-P, IHC-Fr, ICC, Flow Cyt, ELISA
Related conjugates and formulations
概述
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产品名称
驴抗山羊IgG H&L (Cy2 ®)预吸附二抗
参阅全部 IgG 二抗 -
宿主
Donkey -
靶标种属
Goat -
经测试应用
适用于: ICC/IF, IHC-P, IHC-Fr, ICC, Flow Cyt, ELISAmore details -
最小交叉反应
Cow, Guinea pig, Hamster, Horse, Mouse, Rabbit, Rat more details -
免疫原
Full length native Goat IgG (purified).
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偶联物
Cy2 ®. Ex: 489nm, Em: 505nm
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 6.50
Preservative: 0.01% Sodium azide
Constituents: 0.42% Tripotassium orthophosphate, 0.87% Sodium chloride, 1% BSA -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above. -
共轭说明
Cy2 (Cyanine 2-OSu) (Molecular Weight 897 daltons) Absorption Wavelength: 489 nm Emission Wavelength: 505 nm Fluorochrome/Protein Ratio: 3.9 moles Cy2 per mole of Donkey IgG -
克隆
多克隆 -
常规说明
Cy™ and CyDye™ are registered trademarks of Cytiva.
This secondary antibody is specifically designed for the detection of multiple primary antibodies (polyclonal or monoclonal) of different host species in experiments where cells are simultaneously labeled without unwanted cross reaction.
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研究领域
应用
应用 | Ab评论 | 说明 |
---|---|---|
ICC/IF |
1/1000 - 1/5000.
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|
IHC-P |
Use at an assay dependent concentration.
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|
IHC-Fr |
Use at an assay dependent concentration.
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|
ICC |
Use at an assay dependent concentration.
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|
Flow Cyt |
Use at an assay dependent dilution.
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|
ELISA |
1/10000 - 1/50000.
|
说明 |
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ICC/IF
1/1000 - 1/5000. |
IHC-P
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
ICC
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent dilution. |
ELISA
1/10000 - 1/50000. |
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab6948 被引用在 2 文献中.
- Amo L et al. Podocalyxin Expressed in Antigen Presenting Cells Promotes Interaction With T Cells and Alters Centrosome Translocation to the Contact Site. Front Immunol 13:835527 (2022). PubMed: 35711462
- Barsi JC et al. Mind bomb1 is a ubiquitin ligase essential for mouse embryonic development and Notch signaling. Mech Dev 122:1106-17 (2005). PubMed: 16061358