驴F(ab')2 Anti-Rat IgG - H&L (DyLight® 488), pre-adsorbed (ab102232)

概述

  • 产品名称驴F(ab')2 Anti-Rat IgG - H&L (DyLight® 488), pre-adsorbed
    参阅全部 IgG 二抗
  • 靶标种属Rat
  • 特异性By immunoelectrophoresis and ELISA this antibody reacts specifically with rat IgG and with light chains common to other rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to bovine, chicken, goat, horse, human, mouse, rabbit and sheep IgG was detected. This antibody may cross react with IgG from other species.
  • 经测试应用适用于: IHC-P, ICC, Flow Cytmore details
  • 最小交叉反应
    Chicken, Cow, Goat, Horse, Human, Mouse, Rabbit, Sheep more details
  • 偶联物DyLight® 488. Ex: 493nm, Em: 518nm

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明Antiserum was solid phase absorbed to ensure class specificity. Antiserum was cross absorbed using bovine, chicken, goat, horse, human, mouse, rabbit and sheep immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to DyLight® 488.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab102232 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/50 - 1/500.
ICC 1/50 - 1/500.
Flow Cyt 1/50 - 1/200.

Donkey F(ab')2 Anti-Rat IgG - H&L (DyLight® 488), pre-adsorbed 图像

  • Sample: mouse embryonic stem cell-differentiated embryoid bodies (EBs)

    Preparation:

    • Fix in 3%PFA in PBS for 30 min at RT
    • Incubate in 7.5% sucrose-PBS for 3h at RT
    • Incubate in 15% sucrose-PBS at 4 degree Celsius overnight
    • Embed the EBs in tissue-Tek OCT compound
    • Cut frozen sections to 4-20 µm thickness


    Primary antibody: Rat anti-perlecan (basement membrane marker), 1:100
    Secondary antibody: Donkey F(ab')2 polyclonal to Rat IgG - H&L DyLight® 488 (ab102232), 1:200
    Nuclei were counterstained with DAPI

Donkey F(ab')2 Anti-Rat IgG - H&L (DyLight® 488), pre-adsorbed (ab102232)参考文献

This product has been referenced in:
  • Xia F  et al. IRGM1 regulates oxidized LDL uptake by macrophage via actin-dependent receptor internalization during atherosclerosis. Sci Rep 3:1867 (2013). Read more (PubMed: 23689639) »

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"