驴F(ab')2 Anti-Rabbit IgG H&L (FITC) (ab98439)

概述

  • 产品名称驴F(ab')2 Anti-Rabbit IgG H&L (FITC)
    参阅全部 IgG 二抗
  • 靶标种属Rabbit
  • 经测试应用适用于: IHC-P, ICC/IF, Flow Cytmore details
  • 偶联物FITC. Ex: 493nm, Em: 528nm

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明This antibody was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragment were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to Fluorescein.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab98439 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/50 - 1/500.
ICC/IF 1/50 - 1/500.
Flow Cyt 1/50 - 1/200.

Donkey F(ab')2 Anti-Rabbit IgG H&L (FITC) 图像

  • Sample: mouse embryonic stem cell-differentiated embryoid bodies (EBs)

    Preparation:

    • Fix in 3%PFA in PBS for 30 min at RT
    • Incubate in 7.5% sucrose-PBS for 3h at RT
    • Incubate in 15% sucrose-PBS at 4 degree Celsius overnight
    • Embed the EBs in tissue-Tek OCT compound
    • Cut frozen sections to 4-20 µm thickness


    Primary antibody: Rabbit anti-laminin alpha 1 (basement membrane marker), 1:400
    Secondary antibody: Donkey F(ab')2 polyclonal Secondary Antibody to Rabbit IgG - H&L (FITC), pre-adsorbed (ab98439), 1:100
    Nuclei were counterstained with DAPI
    F-actin was stained with rhodamine-phalloidin, 1:100

Donkey F(ab')2 Anti-Rabbit IgG H&L (FITC) (ab98439)参考文献

ab98439 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab98439.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"