驴F(ab')2 Anti-Mouse IgG - H&L (DyLight® 488), pre-adsorbed (ab98766)

概述

  • 产品名称驴F(ab')2 Anti-Mouse IgG - H&L (DyLight® 488), pre-adsorbed
    参阅全部 IgG 二抗
  • 靶标种属Mouse
  • 经测试应用适用于: Flow Cyt, ICC/IF, IHC-Pmore details
  • 最小交叉反应
    Chicken, Cow, Goat, Horse, Human, Rabbit, Rat, Sheep more details
  • 偶联物DyLight® 488. Ex: 493nm, Em: 518nm

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明This antibody was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragment were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to DyLight® 488.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab98766 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt 1/50 - 1/200.
ICC/IF 1/50 - 1/500.
IHC-P 1/50 - 1/500.

Donkey F(ab')2 Anti-Mouse IgG - H&L (DyLight® 488), pre-adsorbed 图像

  • Sample: mouse embryonic stem cell-differentiated embryoid bodies (EBs)

    Preparation:

    • Fix in 3%PFA in PBS for 30 min at RT
    • Incubate in 7.5% sucrose-PBS for 3h at RT
    • Incubate in 15% sucrose-PBS at 4 degree Celsius overnight
    • Embed the EBs in tissue-Tek OCT compound
    • Cut frozen sections to 4-20 µm thickness


    Primary antibodies:
    Mouse anti-disabled-2 (endoderm marker), 1:100
    Rabbit anti-lamin a 1 (basement membrane marker), 1:400

    Secondary antibodies:
    Donkey F(ab')2 polyclonal Secondary Antibody to Mouse IgG -H&L (DyLight® 488), pre-adsorbed (ab98766), 1:200
    Goat anti-rabbit IgG Cy3®, 1:200

    Nuclei were counterstained with DAPI
  • Emission spectra of DyLight® fluorochromes available in our catalog.
    Line colors represent the approximate visible colors of the wavelength maxima.

Donkey F(ab')2 Anti-Mouse IgG - H&L (DyLight® 488), pre-adsorbed (ab98766)参考文献

ab98766 has not yet been referenced specifically in any publications.

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I can confirm it is not unusual for an antibody to be kept at 4oC. The datasheet for many of our antibodies recommend to store at this temperature. At this temperature, they should be stable for at least 1 year. (...

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