驴F(ab')2 Anti-Human IgG - H&L (PE), pre-adsorbed (ab102439)

概述

  • 产品名称
    驴F(ab')2 Anti-Human IgG - H&L (PE), pre-adsorbed
    参阅全部 IgG 二抗
  • 靶标种属
    Human
  • 特异性
    By immunoelectrophoresis and ELISA this antibody reacts specifically with human IgG and with light chains common to other human immunoglobulins. Cross reactivity with IgA and IgM is negligible. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to bovine, chicken, goat, horse, mouse, rabbit, rat and sheep IgG was detected. This antibody may cross react with IgG from other species.
  • 经测试应用
    适用于: Flow Cytmore details
  • 最小交叉反应

    Chicken, Cow, Goat, Horse, Mouse, Rabbit, Rat, Sheep more details
  • 偶联物
    Phycoerythrin. Ex: 488nm, Em: 575nm

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C.
  • 存储溶液
    Preservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    Antiserum was cross adsorbed using bovine, chicken, goat, horse, mouse, rabbit, rat and sheep immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to Phycoerythrin.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab102439 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt 1/50 - 1/200.

Donkey F(ab')2 Anti-Human IgG - H&L (PE), pre-adsorbed (ab102439)参考文献

ab102439 has not yet been referenced specifically in any publications.

Product Wall

Application
Flow Cytometry
CHO cell line expressing membrane bound human TNFα (stable transfectants) was incubated with 10μg/ml Remicade (anti-human TNFa monoclonal antibody) for 1 h in 4°C. The unbound antibody was washed off by centrifugation (300x g for 5min) and binding of remicade was detected with PE Donkey F(ab)2 anti-hIgG H&L (ab102439) – 1:100 (5ug/ml), 30 min incubation in 4°C.

PE donkey F(ab)2 anti-hIgG detected binding of remicade to TNFα CHO cell line giving strong positive signal and displaying minimal non-specific binding the cells alone, non-treated with remicade.
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提交于 Apr 25 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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