驴F(ab) Anti-Rabbit IgG H&L (HRP) (ab102283)

概述

  • 产品名称驴F(ab) Anti-Rabbit IgG H&L (HRP)
    参阅全部 IgG 二抗
  • 靶标种属Rabbit
  • 特异性By immunoelectrophoresis and ELISA this antibody reacts specifically with rabbit IgG and with light chains common to other rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgG from other species.
  • 经测试应用适用于: WB, ELISA, ICC, IHC-Pmore details
  • 偶联物HRP

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C.
  • 存储溶液Preservative: 0.1% Proclin
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明Antiserum was solid phase adsorbed to ensure specificity. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab) fragments were generated using a papain digestion. F(ab')2, Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to HRP.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab102283 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/5000 - 1/30000. (Colorimetric) or 1/10000 - 1/50000 (Chemiluminescence)
ELISA 1/10000 - 1/100000. (Primary)
ICC 1/200 - 1/5000.
IHC-P 1/200 - 1/5000.

Donkey F(ab) Anti-Rabbit IgG H&L (HRP) (ab102283)参考文献

ab102283 has not yet been referenced specifically in any publications.

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We recommend not to store this antibody at -20C. In our experience the conjugated antibodies should not be frozen because the conjugate lost reactivity quite often.

I would suggest thawing the antibody onc...

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Thank you very much for your question.

According to the lab, all of the cysteines in the F(ab) fragment should be in five pairs of disulfide bonds. There should be no free cysteines or thiols unless you do a partial reduction to break some of...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"