驴F(ab) Anti-Goat IgG H&L (TRITC) (ab6522)

概述

  • 产品名称驴F(ab) Anti-Goat IgG H&L (TRITC)
    参阅全部 IgG 二抗
  • 靶标种属Goat
  • 特异性Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein and anti-Donkey Serum. No reaction was observed against anti-Papain or anti-Donkey IgG F(c).
  • 经测试应用适用于: ICC/IF, Flow Cyt, Immunomicroscopy, ELISAmore details
  • 免疫原

    Goat IgG whole molecule

  • 偶联物TRITC. Ex: 547nm, Em: 572nm

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.01% Sodium Azide
    Constituents: 10mg/ml BSA (IgG and protease free), 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • 纯化说明This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation.
  • 共轭说明Tetramethylrhodamine isothiocyanate (TRITC) (Molecular Weight 444 daltons) Absorption Wavelength: 550 nm Emission Wavelength: 570 nm Fluorochrome/Protein Ratio: 1.2 moles TRITC per mole of Donkey IgG Fab
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab6522 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF
Flow Cyt
Immunomicroscopy
ELISA
  • 应用说明Flow Cyt: Use at an assay dependent dilution.
    IM: Use at an assay dependent dilution.

    Suitable for other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Donkey F(ab) Anti-Goat IgG H&L (TRITC) (ab6522)参考文献

    ab6522 has not yet been referenced specifically in any publications.

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