驴Anti-Chicken IgY H&L (FITC) (ab63507)

概述

  • 产品名称驴Anti-Chicken IgY H&L (FITC)
    参阅全部 IgY 二抗
  • 靶标种属Chicken
  • 经测试应用适用于: IHC-Frmore details
  • 免疫原

    Purified chicken IgY isolated from normal chicken egg yolks.

  • 偶联物FITC. Ex: 493nm, Em: 528nm

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C.
  • 存储溶液Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 50% Glycerol, PBS, pH 7.2
  • Concentration information loading...
  • 纯度Protein L purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab63507 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr Use at an assay dependent dilution.

Donkey Anti-Chicken IgY H&L (FITC) 图像

  • ab13970 staining GFP in E10.5 mouse embryo sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with PBS/0.5% Triton X100 and blocked with protein block for 30 minutes at 25°C. Samples were incubated with primary antibody (1/500) for 3 hours at 25°C. A FITC-conjugated donkey anti-chicken IgY H&L (ab63507) (1/500) was used as the secondary antibody.

    See Abreview

  • Ab63507 was used at dilution 1/100 with the primary antibody ab13970 in IHC - Free Floating. See the review on ab13970.

Donkey Anti-Chicken IgY H&L (FITC) (ab63507)参考文献

This product has been referenced in:
  • Ribera A  et al. Biochemical, histological and functional correction of mucopolysaccharidosis type IIIB by intra-cerebrospinal fluid gene therapy. Hum Mol Genet 24:2078-95 (2015). IHC-P . Read more (PubMed: 25524704) »

See 1 Publication for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Cryostat sections of E10.5 mouse embryo, which were fixed with PFA before gelatin/sucrose embedding.
Antigen retrieval is optional in this staining.
Primary antibody: Anti-GFP antibody (ab13970) 1:800 at 4 degree overnight.
Secondary antibody: Donkey Anti-Chicken (FITC) ab63507 at RT 4h (long incubation time of secondary would help to increase the signal).
Username

Abcam user community

Verified customer

提交于 Feb 25 2014

Thank you for contacting Abcam.

I managed to find antibodies against rat GFAP, FOX3, Iba1, and CD68 all raised in different hosts (chicken, rabbit, goat, and mouse, respectively). They each are highly rated by other researchers and have numero...

Read More
Application Immunohistochemistry free floating

Sample: Mouse Tissue sections (Brain and spinal cord)
Specification: Brain and spinal cord

Other product details
Dilution: 1/500
Incubation time: 12 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: PBS

Secondary antibody
Secondary antibody: None used

Additional data
Additional Notes: Please note that the below average score represents a score for an application that was not under the tested applications list on the information sheet.
Abcam response: Thank you for submitting feedback on this antibody in an untested application.
Username

Abcam user community

Verified customer

提交于 Nov 06 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"