Anti-DOK7抗体(ab75049)

概述

  • 产品名称Anti-DOK7抗体
    参阅全部 DOK7 一抗
  • 描述
    兔多克隆抗体to DOK7
  • 经测试应用适用于: WB, IHC-P, Indirect ELISA, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide derived from N-terminus of human DOK7.

  • 阳性对照
    • human brain tissue, HepG2 cells, extracts from mouse brain cells.

性能

应用

Our Abpromise guarantee covers the use of ab75049 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).
IHC-P 1/50 - 1/100.
Indirect ELISA 1/5000.
ICC/IF 1/500 - 1/1000.

靶标

  • 功能Probable muscle-intrinsic activator of MUSK that plays an essential role in neuromuscular synaptogenesis. Acts in aneural activation of MUSK and subsequent acetylcholine receptor (AchR) clustering in myotubes. Induces autophosphorylation of MUSK.
  • 组织特异性Preferentially expressed in skeletal muscle and heart Present in thigh muscle, diaphragm and heart but not in the liver or spleen (at protein level).
  • 疾病相关Defects in DOK7 are the cause of familial limb-girdle myasthenia autosomal recessive (LGM) [MIM:254300]; also called congenital myasthenic syndrome type 1B or CMS1B. LGM is a congenital myasthenic syndrome characterized by a typical 'limb girdle' pattern of muscle weakness with small, simplified neuromuscular junctions but normal acetylcholine receptor and acetylcholinesterase function.
  • 序列相似性Contains 1 IRS-type PTB domain.
    Contains 1 PH domain.
  • 细胞定位Cell membrane. Cell junction > synapse. Accumulates at neuromuscular junctions.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Docking protein 7 antibody
    • DOK 7 antibody
    • DOK7 antibody
    • DOK7_HUMAN antibody
    • Downstream of tyrosine kinase 7 antibody
    • Protein Dok-7 antibody
    see all

Anti-DOK7 antibody 图像

  • Human brain tissue with ab75049 at 1/50 dilution, in the absence and presence of the immunising peptide.
  • HepG2 cells with ab75049 at 1/500 dilution, in the absence and presence of the immunising peptide.
  • All lanes : Anti-DOK7 antibody (ab75049) at 1/500 dilution

    Lane 1 : extracts from mouse brain cells
    Lane 2 : extracts from mouse brain cells with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 53 kDa
    Observed band size : 53 kDa
    Additional bands at : 46 kDa,80 kDa. We are unsure as to the identity of these extra bands.

Anti-DOK7 antibody (ab75049)参考文献

ab75049 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Frozen sections)
Sample Rat Tissue sections (Tibialis anterior muscle)
Specification Tibialis anterior muscle
Fixative Paraformaldehyde
Permeabilization Yes - Triton X-100, 3% in TBS
Username

Mr. Alejandro Gomez

Verified customer

提交于 Sep 10 2012

DISCOUNT CODE: 000000


Expiration date: 05. October 2012

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