Anti-DOK7抗体(ab75049)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to DOK7
  • 经测试应用
    适用于: WB, IHC-P, Indirect ELISA, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide derived from N-terminus of human DOK7.

  • 阳性对照
    • human brain tissue, HepG2 cells, extracts from mouse brain cells.

性能

应用

Our Abpromise guarantee covers the use of ab75049 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).
IHC-P 1/50 - 1/100.
Indirect ELISA 1/5000.
ICC/IF 1/500 - 1/1000.

靶标

  • 功能
    Probable muscle-intrinsic activator of MUSK that plays an essential role in neuromuscular synaptogenesis. Acts in aneural activation of MUSK and subsequent acetylcholine receptor (AchR) clustering in myotubes. Induces autophosphorylation of MUSK.
  • 组织特异性
    Preferentially expressed in skeletal muscle and heart Present in thigh muscle, diaphragm and heart but not in the liver or spleen (at protein level).
  • 疾病相关
    Defects in DOK7 are the cause of familial limb-girdle myasthenia autosomal recessive (LGM) [MIM:254300]; also called congenital myasthenic syndrome type 1B or CMS1B. LGM is a congenital myasthenic syndrome characterized by a typical 'limb girdle' pattern of muscle weakness with small, simplified neuromuscular junctions but normal acetylcholine receptor and acetylcholinesterase function.
  • 序列相似性
    Contains 1 IRS-type PTB domain.
    Contains 1 PH domain.
  • 细胞定位
    Cell membrane. Cell junction > synapse. Accumulates at neuromuscular junctions.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Docking protein 7 antibody
    • DOK 7 antibody
    • DOK7 antibody
    • DOK7_HUMAN antibody
    • Downstream of tyrosine kinase 7 antibody
    • Protein Dok-7 antibody
    see all

图片

  • Human brain tissue with ab75049 at 1/50 dilution, in the absence and presence of the immunising peptide.
  • HepG2 cells with ab75049 at 1/500 dilution, in the absence and presence of the immunising peptide.
  • All lanes : Anti-DOK7 antibody (ab75049) at 1/500 dilution

    Lane 1 : extracts from mouse brain cells
    Lane 2 : extracts from mouse brain cells with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 53 kDa
    Observed band size : 53 kDa
    Additional bands at : 46 kDa,80 kDa. We are unsure as to the identity of these extra bands.

文献

ab75049 has not yet been referenced specifically in any publications.

客户评价及客户问答

Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (Tibialis anterior muscle)
Specification
Tibialis anterior muscle
Fixative
Paraformaldehyde
Permeabilization
Yes - Triton X-100, 3% in TBS
Username

Mr. Alejandro Gomez

Verified customer

提交于 Sep 10 2012

DISCOUNT CODE: 000000


Expiration date: 05. October 2012

I am very pleased to hear you would like to accept our offer and test ab75049 in IHC-Fr. This code will give you: the value of ab75049 a future order before the expiratio...

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